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1. This method can be used to serilize a liquid media without harmingheat labile ingredints:a. autoclavingb. pasteurizationc. freezingd. cold filtratione. addition of ethylene oxide to the media2. Consider two parallel cultures. They are in all ways identicalexcept that one contains test substance X. You observe that abacterium you are studying grows equally well in both media.Therefore, test substance X is ___________.A macronutrientA micronutrientAn essential nutrientA toxinNot a nutrient3. A microbe uses CO2 as its sole source of carbon and oxidizes H2 asits source of energy, to which nutritional group does this microbebelong?PhotoautotrophChemoorganotrophPhotoorganotrophChemoautotroph4. A mutant bacterium does not produce a functional hydroxamate. Thisbacterium would have difficulty obtaining ___________.PhosphateIronAmmoniaThiosulfateHydrogen sulfide5. A medium containing methylene blue and incubated at 55º Cenriches for ______.Mesophilic mycoplasmaHyperthermophilic gram negativesGram positivesThermophilic gram negativesThermophilic gram positives6. Bacterium “A” has a growth rate constant of 4doubles/hour. Bacterium “B” has a generation time of0.25 hours/double. Which bacterium grows faster?“A” grows faster than “B”“B” grows faster than “A”A” and “B” grow at the same rateNo conclusion can be made from this data7. Assume you inoculated 100 cells into 100 ml of nutrient broth. Youthen inoculated 100 cells of the same species into 200 ml ofnutrient broth. After incubation for 12 hours, you should haveMore cells in the 100 mlMore cells in the 200 mlThe same number of cells in both.8. _________ halodurics use K+ as their osmolyte.AllSomeNo9. A medium containing 3.2% NaCl and 0.025% phenethyl alcohol wouldenrich for _________.NonhalophilesGram-negative haloduricsGram-negative halophilesGram-positive hyperhalophilesGram-positive halophiles10. The value of the minimum inhibitory concentration dependsupon_________.The microbe’s intrinsic sensitivity to the antimicrobialThe rate at which the antimicrobial diffuses through the agarBoth A and BNeither A nor B11. Which enzyme would be used to neutralize the activity of the[OH·] free radical?Superoxide dismutaseCatalasePeroxidaseNitrogenaseNone of the above12. Which of the following can sterilize?70% ethanol10% sodium hypochorite solutionEthylene oxideMecurochromeAll of the above13. You are developing a new line of protease containing laundrydetergent. You plan on marketing the detergent to the NFL to removegrass and blood stains from their uniforms. You most likelyisolated the protease from which group of bacteria?AcidophileNeutrophileAlkalophileStrict anaerobesNon-halophile14. True or False: Fungi are better osmophiles than most bacteria.TrueFalse15. The cell density of population of microbes expands six-thousandfold. Approximately how many times did the population double?91012141616. Thirty-six colonies grew on nutrient agar plate from 500μl ofundiluted sample in a standard plate count. How many cells / mlwere in the original sample?17,000900183672
A large capacitance of 2.00 mF is needed for a certain application. What is the maximum voltage that can be applied
What are Engineer As obligations under these circumstances - Review the engineering code of ethics.
A bedroom bureau with a mass of 57 kg, including drawers and clothing, rests on the horizontal floor. What is the static friction force on the bureau initially
The density of water is 100g/ml at 4 degrees celsius. How many water molecules are present in 2.56ml of water at this temperature
A 800 kg car pushes a 2000 kg truck that has a dead battery. What is the magnitude of the force of the truck on the car
A 4800 kg truck is parked on a 14° slope. How big is the friction force on the truck
What limits must be placed on the value of x so that all concentrations are positive. By putting the equilibrium concentrations (in terms of x) into the equilibrium-constant expression, derive an equation that can be solved for x.
Using sickle-cell anemia as a basis, describe what is meant by amolecular or genetic disease. What are the similarities anddissimilarities between this type of disorder and a disease causedby an invading microorganism?
As a semi-confluent monolayer, lets say that of Vero cell lineis washed or treated with PBS(phosphate buffered saline) to removecell debris. What is the mechenism through which the contentsof PBS remove cell debris?
Two parallel conducting plates are separated by 10.0 cm, and one of them is taken to be at zero volts. What is the voltage between the plates
A plant cell is placed in a solution in a flask with a solute potential of -0.6MPa. When the cell and flask reach equilibrium, the cell's pressure is +0.5. At equilibrium, what is the solute potential of the cell?
Which type of transport functions to make a concentration gradient ?
describe the first step involved in replication of each genome. Your description can include a diagram and should cover.
A study of mRNA isolated from human brains, post mortem, has been used to analyze genes that may give rise to inherited mental conditions. What is a possible problem with this method. Explain.
Two genes, designated A and B, are located 10 mu from each other. A third gene, designated C, is located 15 mu from B and 5 mu from A. A parental generation consisting of AAbbCC and aaBBcc individuals were crossed to each other.
Discuss and explain the cis and trans acting regulatory elements and give examples of each?
Imagine a protocol that requires you make a 50mL sample of 0.6 M cola. You know that the concentrated syrup is at 3M. Map out your procedure for making this dilution. Use carbonated water as your diluent.
Briefly explain the Human Genome in terms of PURPOSE, number of genes,and chromosome pairs. (please make this simple)
Draw a phospholipid containing palmitic and linoleic acid with the unsaturated fatty acid at C-2 of glycerol. To the phosphate, esterify a molecule of choline. Indicate which portion of the molecule would be in the lipid bilayer and which would be..
When a nerve fiber is polarized, the concentration of - A. sodium and potassiumions is higher on the inside of its membrane
Pyrolase, is a cellulase isolated from a hyperthermophilic archeon that grows in a hydrothermal vent and is used in which industry?
Are they necessarily required or simply an artefact of the evolutionary history of eukaryotes. Why, for example, would eukaryotic cells require a membrane-bound nucleus when prokaryotes seem to replicate efficiently without one.
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