Separate the denatured protein into several components

Assignment Help Biology
Reference no: EM13152809

Suppose you suspect that there is more than one type of subunit in the native molecule. So you treat the native molecule with mercaptoethanol and urea and try to separate the denatured protein into several components. If there really are 2 (or more) different types of subunits, which of the following methods is most likely to separate them? (dialysis) (gel filtration) (paper chromatography) (column chromatography based on solubility) (gel electrophoresis) (hydrolysis) (any of these should work)

Reference no: EM13152809

Questions Cloud

Describes change in supply or change in quantity supplied : The success of the apple ipad leads more firms to begin producing tablet computers. In the six months following the japanese earthquake and tsunami in 2011, production of automobiles in japan declined by 20 percent.
What is the pressure in the bottle : An empty 2.0 L soda bottle is tightly capped at 25 degrees C and 732 mmHg. If the bottle is heated to 100 degrees C what is the pressure in the bottle
Sharing of the pre-bonus income : The partnership contract for Hanes and Jones LLP provides that Hanes is to receive a bonus of 20% of net income (after the bonus) and that the remaining net income is to be divided equally. If the partnership income before the bonus for the year i..
Photography equipment in the business : Prepare general journal entries for the following transactions of a new business called Pose for Pics. Aug. 1 Hashim Paris, the owner, invested $7,500 cash and $32,500 of photography equipment in the business.
Separate the denatured protein into several components : Suppose you suspect that there is more than one type of subunit in the native molecule. So you treat the native molecule with mercaptoethanol and urea and try to separate the denatured protein into several components.
Calculate the number of moles of h2o : Starting with 0.86 moles of HF, calculate the number of moles of H2O that is produced by this balanced reaction.
Is compound symmetry a reasonable model : The book has a program on page 153 for this, but the program was written is an unusual way. The subject should be mouse. So how should we dene mouse in the data?
Classifications of net assets established by fasb statement : What are the three classifications of net assets established by FASB Statement 117?  How are these net assets affected by the existence or absence of donor-imposed restrictions? How are releases of these net assets accomplished?
What is the molar mass of lysine : Lysine is an amino acid that is an essential part of nutrition but which is not synthesized by the human body.

Reviews

Write a Review

Biology Questions & Answers

  Determine the percentage of individuals in this population

The following Punnett square shows the inheritance of the sickle cell and SRY genes, with X representing the X chromosome with no SRY gene and Y representative the Y chromosome which does have an SRY gene. Complete the Punnett square.

  How many bacterial cells

How many bacterial cells would you expect to see if you plated the entire solution on one Ampicillin-treated plate and incubated them for one hour?

  The most likely defect associated with the ser63 deletion

What is the most likely defect associated with the Ser63 deletion mutant. How would you design an experiment to test your hypothesis using culture cells and the expression construct of wild-type and Ser63 deletion mutant of P0.

  What is the association constant for the peptide

The following kon and koff rates for the interaction of antibody (IgG) and a 40 amino acid peptide were determined via surface plasmon resonance (a Biacore machine) to be: kon = 210 nM-1·sec-1, koff=11,000 sec-1.

  Which of the following is direct product of dna replication

Which of the following is the direct product of DNA replication.

  What is the aim of ferementation after glycolysis

Why use starved starved yeast rather than sugar fed yeasts for fermentation.What is the aim of ferementation after glycolysis.

  What skull features do prey animals have in common

What skull features do prey animals have in common.

  Which of the following neoplsia has been link to a delition

Which of the following neoplsia has been link to a delition of a tumor suppressor gene?

  Aliquot and diluent volumes

Discuss how much sample what sized aliquot is required to prepare 500 mL of a 1 to 100 dilution, and how much diluent would you need?

  Why are complement binding sites found on red blood cells

Why are complement binding sites found on Red blood cells and what is the result of their binding?

  Describe how ca2+ levels in the blood are regulated

Describe how Ca2+ levels in the blood are regulated by hormones?

  Draw the structure of a plant cell

Draw the structure of a plant cell, label the parts and include the major biological molecules found in such cell referring to specific parts. Include the functions of each structure.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd