Scanning microscopy or transmission electron microscopy

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Reference no: EM133628344

It is possible that you recently discovered a new species of plant in the Amazon Rainforest. It is surprising that this plant can survive with limited sunlight in the diverse ecosystem of the Amazon. The plants unique quality has the potential to have a significant impact on both the realm of ecology and diverse sectors. Your main goal is to study how well plants use sunlight to make food. You will looking at how the plants cells, called chloroplasts, look at where the pigments (chlorophyll and carotenoids) are, and using microscopes.

1. Which method is better for looking at the structure of chloroplasts in this plant, confocal scanning microscopy or transmission electron microscopy. Consider the principles, advantages, and limitations of each method and align them with the goals of your research to justify your answer.

2. Both conventional fluorescence microscopy (FM) and CSM possess the capability to identify the distribution of pigments, specifically chlorophyll and carotenoids. How does the process of stimulating fluorescence and detecting fluorescence differ between these two microscopy techniques? Given these disparities, which approach is more likely to yield superior outcomes for your research? Could you please explain, taking into consideration the specifics of your investigation?

Reference no: EM133628344

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