Restriction digests and gel electrophoresis

Assignment Help Biology
Reference no: EM13534605

Why is it improtant to use purified dna samples in restriction digests and gel electrophoresis (i.e. rather than just using homogenized tissues)?

Reference no: EM13534605

Questions Cloud

Draw a model of DNA replication : Draw a model of DNA replication for the following DNA strand.: Indicate which strands are old, which strands are new, which strands are template strands, the 5’ and 3’ ends of each strand, the direction of synthesis on each strand and the direction t..
Explain why restriction enzyme and gel electrophoresis : Explain why restriction enzyme and gel electrophoresis are valuable tools in the molecular biology lab. What factors (aside from size) could influence the migration speed of dna fragments in gel electrophresis? The respiratory centers that control ve..
Two people using breathing gear under water : Imagine that you have two people using breathing gear under water. Diver 1 is using an old style "rebreather" that removes carbon dioxide before the mixture is "rebreathed" and therefore decreases overall carbon dioxide concentrations in the blood..
Consider a reaction mixture of lambda dna and EcorRl split : Consider a reaction mixture of lambda dna and EcorRl split into 3 tubes. tube#1 is allowed to sit for an hr at room temp. tube#2 is incubated at 37 degree celuius, while tube#3 is incubated at 60 degree celuius for an hr. when these sampes are ele..
Restriction digests and gel electrophoresis : Why is it improtant to use purified dna samples in restriction digests and gel electrophoresis (i.e. rather than just using homogenized tissues)?
What is the purpose of restriction endonucleases in prokaryo : what is the purpose of restriction endonucleases in prokaryotes? how do these organisms protect their dna against the effect of their own restriction enzymes?
Theory of gradualism andpuntuated equilibrium : What is the difference between the theory of gradualism andpuntuated equilibrium? What is adaptive radiation? How does adaptive radiationincrease species diversity?
How is cell division controlled : How is cell division controlled, Do all cells continuously divide, why or why not What are the ramifications of uncontrolled cell division?
What is the function of the replication fork : What is the function of the replication fork? Phosphatidylinositol4,5-bisphosphate (PIP2) is an example of phosphoacylglycerol.

Reviews

Write a Review

Biology Questions & Answers

  Q what differences in detail do you notice in freshly

q. what differences in detail do you notice in freshly prepared versus permanently mounted tissue? how did the steps of

  What are the primary mechanisms the body uses

What are the primary mechanisms the body uses to absorb carbohydrates? (i.e., transport mechanisms)?

  Indicate the percentage of the time each possible codon

in a mixed copolymer experiment using polynucleotide phosphorylase, 3/4g:1/4c was used to form the synthetic message. the amino acid composition of the resulting protein was determined to be: indicate the percentage of the time each possible codon..

  Primitiveatmosphere of earth

What condition would have made the primitiveatmosphere of Earth more conducive to the origin of life than thepresent one? The primitive atmosphere

  Consider list of scientists that have awarded nobel price

Consider the list of scientists that have been awarded a Nobel Price for their work in immunology which one would you consider being the most important work and why?

  Describe the growth conditions

You are working with a Gram positive bacterium that can be grown in Tryptic Soy Broth, a complex medium, or in a defined medium containing glucose, ammonium sulfate, potassium phosphate, ferric chloride, sodium phosphate and magnesium sulfate.

  Consider the natural environment or biome found in the

consider the natural environment or biome found in the geographic area where you currently live. for example if you

  The enzyme papain is a thiol protease

The enzyme papain is a thiol protease, using a mechanism similar to the mechanism used by chymotrypsin, except that there is an active site Cys in papain carrying out the catalytic role played by the active site Ser of chymotrypsin.

  Describe the benefits and risks of using biotechnology use

discuss the benefits and risks of using biotechnology use an example to demonstrate the advantages and the potential

  Depolarization of postsynaptic neuron

Suppose that each excitatory presynaptic neuron causes a 0.5mv depolarization of postsynaptic neuron, and each inhibitory presynaptic neuron causes a 0.5mv hyperpolarization of postsynaptic neuron.

  Comparing calls of the two species

Male frogs give calls that attract female frogs to approach and mate. Researchers reviewed mating calls of closely related tree frogs in South America.

  Which is normally a soluble resident of the er lumen

If you were to remove the ER retrieval signal (KDEL) from protein disulfide isomerase (PDI), which is normally a soluble resident of the ER lumen, where would you expect the modified PDI to be located? Why?

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd