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You are doing a PCR that requires 1.6ng of DNA in 2uL of sample. You have 100uL of stock DNA with a concentration of 4ng/ul. Using reasonable pipetting volumes (ex. don't use too much of your sample but don't pipette an unreasonably small volume), how would you dilute your stock DNA sample to meet this criteria? Use the procedure in Exercise #3; show all formulae, variables, units and calculations
What is a multiplex PCR? What differs in this reaction from a traditional PCR?
How are PCR products fluorescently labelled in the Identifiler Kit? Name four dyes used in this kit and their corresponding colours.
What standard is used during electropherogram interpretation to determine the size (in bp) of a peak given its electrophoretic mobility?
What reference standard is used during electropherogram interpretation to score alleles given the size of a DNA fragment?
Define incomplete 3' addition. How does this appear on an electropherogram?
What causes stutter? In the Identifiler kit, what is the most common size difference (in bp) between the true allele and the resulting stutter product? What does this difference represent?
Each of the following objects has a radius of 0.174 m and a mass of 2.79 kg, and each rotates about an axis through its center, Find the magnitude of the angular momentum of each object
An electric dipole moment of p = (5, -6, 4.5) Cm is inside a uniform electric field of E = (2.25, -6, 7.75) V/m. What is the potential energy of the dipole
a positron is a particle with the same mass and magnitude charge as an electron, but a positron has a positive charge. what is the system's total kinetic energy
Dynamo theory, inner core, outer core, convection, and rotation. The Earth's magnetic field undergoes periodic reversals. How was this discovered, and by whom? What is the current best theory of its cause? How does the magnetosphere interact with cha..
In a ballistics test, a 21 bullet traveling horizontally at 1400 goes through a 25--thick 370 stationary target and emerges with a speed of 930 . The target is free to slide on a smooth horizontal surface. What is the target’s speed just after the bu..
two capacitors are connected in parallel across the terminals of a battery. one has a capacitance of 2.0 microf and the
A gold prospector finds a solid rock that is composed solely of quartz and gold. Find the mass of the gold in the rock
question if a soap bubble is 140 nm thick what wavelength is most strongly reflected at the center of the outer surface
A golfer hits a shot to a green that is elevated 3.0 m above the point where the ball is struck. find the speed of the ball just before it lands
A 2.95 kg fish is attached to the lower end of a vertical spring that has negligible mass and force constant 890 N/m. What is the maximum speed of the fish as it descends
There is evidence that elephants communicate via infrasound, generating rumbling vocalizations as low as 14 Hz that can travel up to 10 km. Determine the intensity level of the infrasound
A bullet of mass 180 gram is fired horizontally from a gun with a velocity of 675 meter per second into a wooden block of mass 6.0 kilogram sitting on a horizontal table. Determine the velocity of the block after being hit by bullet
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