Question relates to genome sequencing using the shotgun

Assignment Help Biology

Reference no: EM13906990

Question relates to genome sequencing using the shotgun method.

When presented with the sequence contigs, I was taught that to fill in physical gaps between contigs, we can use PCR.

"Pairwise combinations of contig end primers can be used to PCR the organism's genome. An adjacent pair of primers will give a PCR product of

the size of the gap between them."However, why is this? Why do adjacent pairs of primers give a PCR product, but non-adjacent pairs of primers would not? My understanding is that the pairs of short primers we using are both complementary to a single strand of DNA from the organism's genome.

Reference no: EM13906990

Questions Cloud

Develop a basic android weather application : Develop a basic Android "Weather Application". Your application should have a single view with a list of cities (see below) and the current temperature at that location.

How many copies should be sold to general readers : Describe why there might be this difference in demand between the two types of readers. How many total copies of the Wall Street Journal should be printed?

What is the role of insurance payers in the quality : What is the role of insurance payers in the quality of services

Calculate the amount of gross profit from the transaction : On November 1, 2015 Wrenns Martch sold merchandise for $10,000, FOB destination, with payment terms of 3/10, n/40. Sales returns on this sale amounted to $3,000. Wrenns received payment for the balance on November 10, 2015. The cost of goods sold was..

Question relates to genome sequencing using the shotgun : Question relates to genome sequencing using the shotgun method.

Affected if these transfers were downstream : A worksheet is being developed to consolidate Williams, Incorporated, and Brown Company. These two organizations have made considerable intercompany transactions. How would the consolidation process be affected if these transfers were downstream? How..

What was the ethical dilemma in case? : What was the ethical dilemma in case?

Prepare schedule of significant noncash investing : Use the following company information to prepare a schedule of significant noncash investing and financing activities:

What are some of the flaws in this observational analysis : Is coffee the key to success in a f2f Stat 200 class? (Would it be necessary in our on-line course?) FINALLY, what are some of the flaws in this observational analysis? How might it be better designed

User Account

All Pages