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1. The recipe for 10x TBE buffer is 890 mM Tris, 890 mM boric acid, 20 mM EDTA. What is the concentration of 1x TBE buffer?
2. A recipe calls for 10 ml of a 25mM stock of EDTA. However, all you have on hand is a 500 mM stock. How much more concentrated is what you have relative to what the recipe calls for? (i.e. what is the relative concentration).
3. The protocol for transforming Chlamydomonas requires 300 ul of a culture at a density of 1x108 cells/ml. The culture you have is at 1x106/ml. What is the concentration of your culture relative to what the protocol calls for?
When cloning a gene into an expression vector, people frequently use two different restriction enzymes so that passenger DNA can be inserted in the correct orientation with respect to promoter sequence present in the plasmid. But orientation is almos..
explain Why is it advantageous for citrate the product of reaction 1 of the TCA, to inhibit phosphofructokinase, which catalyses the third reaction in glycolysis.
A species has a diploid number of 2n. Meiosis I fails during spermatogenesis, cytoplasm does not divide and only one daughter cell is manufactured. The resulting sperm fertilizes a normal ovum.
Assume you are creating a PCR reaction mixture with a total volume of 50µL. The primer set concentration is 1µl primer/10µl reaction volume.
define the difference between a tumor suppressor and an oncogene with respect to the actual causes of cancer?
What is the yield of ATP when each of the following substrates is completely oxidized to CO2 by a mammalian cell homogenate.
Discuss similarities and differences of the composition and structure of bacterial and eukaryotic ribosomes and identify the features of bacterial ribosomes that are the same for eukaryotic ribosomes
State the rate of growth of population of bacteria? State the initial population at time t = 0 hours?
This job names three DNA features that are required to make a green fluorescent protein (GFP) reporter construct and indicates which of these controls the tissues in which GFP will be expressed if the construct is used to make a transgenic mouse.
Describe why morphine, pentazocine, and naloxone have very different pharmacological and adverse effects on a patient.
Determine which biological macromolecule is not really a polymer and Which statement about biological polymers and their precursors below is TRUE?
Reach capabilities in the sagittal plane can be determined graphically using anthropometric data as shown in the figure on the given page.
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