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Question 1. Acetylation of key proteins is known to correlate with the gene expression in eukaryotes. What class of chromatin proteins is changed? What is the chemical nature of the acetylation that accounts for the alternation in a physical and structural sense? What features-interactions of DNA are altered? How could one check segments of DNA in genes that were altered due to this phenomenon? What is the link between the modification and DNA sequence specificity?
Question 2. Compare and contrast gene expression in prokaryotes vs. eukaryotes from the standpoint of promoter sequences, complexes and RNA polymerase(s). What features are shown in each that are distinct? What proteins, other than RNA polymerases are involved? What is known about the mechanism and ordering of gene expression in eukaryotes that takes it distinct from prokaryotic systems? Be specific, complete and structurally oriented in your answer as possible.
Question 3. Gene inactivation correlates with methylation of DNA. What are the chemical and structural features of this process? How do pleuripotent cells differ from tumor cells in terms of the degree of methylation? Will you rationalize these differences in terms of known biochemical processes thought to be involved in the normal elimination of cells for the benefit of the organism?
Discuss and describe three differences in transcription between prokaryotes and eukaryote and also explain two molecular mechanisms for allelic variation.
Using bacteriophage P22, you performed a three-factor cross in Salmonella typhimurium. The cross was between an Arg-Leu-His- recipient bacterium and bacteriophage P22, which was grown on an Arg+ Leu+ His+ strain. You selected for 1000 Arg+ transdu..
Through writing Henderson hasselback equation describe how pH is equal to pkA when exactly one half of lactic acid is dissociated into H+ and A- ions through titrating with 0.5M equivalents of NAOH.
Benzyl penicillin is absorbed from the human digestive tract by a H+/penicillin symporter that cotransports these 2 species in a 1:1 ratio from the intestinal lumen into the brush border epithelial cells.
he clinical laboratory returns the following arterial blood values for patient: pH = 7.2; plasma HCO3- = 29 mmol/L; plasma pCO2 = 75 mmHg. Determine the patient's acid-base disorder?
How does changing the pH or temperature of a reaction affect the active site of an enzyme?
The mean commute time in the metro New York area is 45 minutes with a standard deviation of 15 minutes.
Upon your return to school after a long summer break ,you are anxious to get back to work in the research lab and complete the last experiment needed for your first publication.OOPS.
What kind of microorganism is selected for on the SAB plate and the 3M Petrifilm?
You're an international athlete. For breakfast you had a lemon poppyseed muffin. Now theres a knock at your door. It is the internation no drugs in athletes committee. They want a sample of your urine for a drug test.
Which of the physical forces can penetrate water most effectively and can thus be used to locate things in the ocean
Why is there a range of total ATP that can be produced through the process of glycolysis, the Krebs cycle, and oxidative phosphorylation?
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