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Q1. An experiment is designed to learn the mechanism of sucrose uptake by plant cells. Cells are immersed in a sucrose solution and pH of the solution is monitored. Samples of the cells are taken at intervals and their sucrose concentration is measured. Their sucrose uptake correlates with the rise in the solution's pH. This rise is proportional to the starting concentration of sucrose in the solution. A metabolic poison that blocks the ability of cells to regenerate ATP is found to inhibit the pH changes in the solution. Recommend a hypothesis accounting for these results. Suggest an experiment to test it.
Q2. Could intelligence be primarily a matter of having plenty of the right neurotransmitter at the correct synapses?
Describe three common types of secondary structural elements found in many globular proteins. What are the prominent forces that are involved in maintaining these structures? Would the same forces apply if the protein were dissolved in a non-aqueo..
How many bacterial cells would you expect to see if you plated the entire solution on one Ampicillin-treated plate and incubated them for one hour?
There are 100 students in your science class and you decide to do a research project focused on the distribution of alleles for brown versus blue eye color. You examine everyone and find out that 64 students have brown eyes and 36 have blue eyes. ..
Describe how RFLPs can be used in DNA fingerprinting? How does this compare to the use of VNTRs in DNA fingerprinting?
A standard procedure in purifying tubulin is to extract the tissue on ice, then add GTP and warm the tissue extract to 37°C. The warmed extract is then centrifuged at a speed insufficient to pellet average sized proteins, but sufficient to pellet ..
Characteristics of a malignant state include: 1) Dysregulation of the cell cycle and 2) Inhibition of apoptosis. These mechanisms are also hallmarks of a many viral infections? Why are these characteristics also beneficial to many viruses? Explain ho..
Mother Nature uses a "fail-safe system" to prevent inappropriate activation of the immune system. Provide several examples of this strategy.
The expression and purification of rGFP started with the increase of the bacterial culture and ended with elution of rGFP off the Ni+2 agarose coloumn.
Certain patients have a genetic disorder affecting a protein that provides support to the mitochondria of all the cells in the body. Yet the patients die of heart attacks.
Determine the major difference between the cytoskeleton of bacteria and eukaryotic cells and if you were to analyze a DNA sample from a eukaryotic cell.
Cancer patients undergoing chemotherapy are normally more susceptible to infections. However, a patient receiving an antitumor drug that inhibited cell division was resistant to salmonella. Provide a possible mechanism for the resistance.
evaluate the melting temperature of the original strand. Because G binds to C more strongly than A binds to T, the melting temperature should increase with the fraction of G/C pairs along the backbone of a sequence.
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