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Q. Suppose a mammalian genome (4x10^9bp in size) is digested with Hind III, and you are cloning a unique fragment of 2kb.
A. Approximate how numerous clones you will need to screen?
B. If you first size select the genomic DNA, realizing a 10-fold purification, and clone that DNA in a plasmid, now how many clones will you need to screen?
C. If you transform bacteria with the plasmid library made in part b above, divide the transformatnts into 20 aliquots and find one has the insert of interest, now how many clones do you need to screen?
D. If you screen 5,000 colonies on a 15cm dish, how various dishes will you require to find a clone of interest? "
In octopi, stubby tentacles (S) is dominant to normal tentacles. This trait also shows reduced penetrance- 90% in homozygous SS and heterozygote. In a cross between two octopi heterozygous for this trait, which proportion of the progeny will have ..
While rummaging through the pantry, a 14 year old boy found a can of spam. He proceeded to eat the canned meat without thorough heating.
show herneation of the tonsils of the cerebellum into which anatomic space.
Which of the following is NOT the way proteins are transported through the Golgi? Clathrin-coated vesicles bud from the ER and fuse with the Golgi.
You're an international athlete. For breakfast you had a lemon poppyseed muffin. Now theres a knock at your door. It is the internation no drugs in athletes committee. They want a sample of your urine for a drug test.
Assume that flower color is inherited by simple dominance and that red flowers are dominant to white flowers.
This question concernsthree E. coli genes. Genes a and b eachencode polypeptides of 300 amino acids, while gene cencodes a polypeptide of 3,000 amino acids, Thus the mutation frequency for gene a is 1 in 10,000
The sequence below represents the middle portion of a gene (so don't look for primer or start sites). A frameshift mutation between base +10 and +11 adds a guanine between the two "c"s in the minus strand of DNA.What functional change in the prote..
Which has the greatest affinity for electrons.
What happens to the chromosome number comparing the beginning and end of meiosis, and why is this essential to the survival of sexually reproducing organisms
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find examples of countries for each population growth model (rapid, slow, zero). Use examples other than the ones described in the text or the lecture. What evidence can you find to support your choices?
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