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Q. A standard procedure in purifying tubulin is to extract tissue on ice, after that add GTP and warm the tissue extract to 37°C. The warmed extract is next centrifuged at a speed insufficient to pellet average sized proteins, but enough to pellet large protein complexes. After centrifugation, the supernatant is discarded, and the pellet is redissolved in cold buffer. GTP is again added to the redissolved pellet proteins, and the solution is again warmed for a few minutes. Then centrifugation at similar speed described above is again performed. This process is repeated several times.
How will this procedure result in purification of tubulin?
Many scientists were reluctant to accept the results of Avery, MacLeod, and McCarty's experiments showing that DNA (rather than protein) was the transforming principle. Why?
What is the skene's gland? Understand the relationship this gland may have with the vagina, urethra, and female ejaculation.
The plasma membrane acts as a barrier in cells of all types to control the movement of substances into and out of the cell. a) Explain how the plasma membrane acts as a semi-permeable barrier to various types of molecules based on its molecular st..
The sequence below represents the middle portion of a gene (so don't look for primer or start sites). A frameshift mutation between base +10 and +11 adds a guanine between the two "c"s in the minus strand of DNA.What functional change in the prote..
Explain the three kinds of the mammals based upon how their young grows.Explain briefly the functions of sensory neurons, inter-neurons, and motor neurons in nervous system.
What factors of the environment and/or biology of the birds pictured lead to similarities/ differences?
The daughter of a colorblind man called John, who herself has normal vision, has a colorblind husband. What are the odds that the first child of the pair will be a boy with normal vision? What about the chances that their daugthers will be colorbl..
Selected species Genus and species Time frame and geographic area
when you incubate the cells in 42 C water and then immerse them in ice, what is this process called and how how this cause transformation? why is this a shock to bacteria? What temperature is that in Fahrenheit?
Amino acid compositions can be determined by heating a protein in 6 M HCL and running the hydrolysate through an ion-exhange column. If you were going to do an amino acid sequencing experiment, why would you want to get an amino acid composition f..
By some estimates there are only a couple of thousand great white sharks alive in the wild. Assume that in the frequency of LDH alleles in a sample of 200 adult sharks in a population off of Australia.
Plasma proteins do not ordinarily enter the glomerular filtrate. In the presence of pathology that makes the glomerulus leaky, plasma proteins can be filtered with serious consequences for renal function, and also for other functions of the body.
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