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Q. A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is additional to a mixture containing EcoRI and bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and after that added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics.
Bacteria contain a plasmid into which eukaryotic gene has integrated will grow in what?
Approximation the age group and gender (where possible) of the individuals in this find. Throughout exposure to elevated glucose, a yeast cell's membrane glucose transporters will rapidly enhance so the cell can import glucose. Which will represent t..
Create a BIO 113 Labe Report based on a experiment we did on the variances in photosynthtic activity in the Elodea plant.
You decide to climb Mt Everest and someone tells you it takes 2-months. Estimate the number of times you would stop and explain why you need to acclimatize. Describe all the physical effects on your body and the dangers.
What are the phenotypes for all possible blood types and the probability of each? Explanation is needed.
In flies, a new mutant male is found with short stubbly bristles instead of normal long bristles. The mutant is mated to a normal female.
What is the probability that the first child of parents who are heterozygous for both the tickle trait and the trembling ears trait will have tickle syndrome but lack trembling ears?
What fraction of demes in each set is likely to become fixed for allele A1 versus A2. Explain the differences in head movement available to fish.
What are the differences between 70s and 80s ribosomes.
A sample of a gas occupies 1.40 x 103 mL at 25oC and 760 mmHg. What volume will it occupy at the same temperature and 380 mmHg?
Explain the challenges and advantages of having internal gills over external gills for gas exchange and explain how increased stress levels can affect hormones which ultimately inhibit the immune system? Include all relevant glands and hormones in ..
At what rate does glucose enter a cell by passive diffusion alone? Estimate a typical cell as an ideal sphere with a diameter of 20 μm. Remember that the surface area of a cell is 4πr2. The permeability coefficient (P=KD/x) for glucose is 3×10..
A 70-year old woman presents with a one hour history of crushing substernal chest pain. Shortly after admission, the patient expires.
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