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Q. A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to the mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in tetracycline resistance gene. This mixture is incubated for numerous hours, exposed to DNA ligase, and after that added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using the technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics.
Bacteria containing a plasmid into which the eukaryotic gene has integrated will grow in what?
calculate a chi-square test statistic to see whether or not the observed progeny phenotypes are significantly different from the expected progeny phenotypes
What process is responsible for position effect variegation? A. Inactivation of a gene due to deletion B. Activation of a gene by an enhancer C. Gene duplication D. Inactivation of a gene due to its incorporation in a heterochromatic region of the..
Find an instance of ecological mess that has taken place in Ontario. Outline the history of the problem, what caused it, what is being done to resolve it. What could have been done differently.
Transcranial magnetic stimulation (TMS) is a noninvasive technique used to stimulate regions of the human brain. A small coil is placed on the scalp, and a brief burst of current in the coil produces a rapidly changing magnetic field inside the br..
Genetic variability is beneficial for a species because it enhances its ability to adapt to changing conditions. Why then does a cell go to great lengths to assure the fidelity of DNA replication?
How humans populated the globe is a highly-debated question among paleoanthropologists. In 5 sentences or less, compare the "Out of Africa" theory to the "Multiregional Theory".
What is the difference between geometric and exponential growth and what factors limit exponential growth (carrying capacity) resulting in logistic growth?
At the start of July classic car wax company had beginning Work in Process of 2,500 units that were 90% completed with respect to matherial and 45% completed with respect to conversion costs.
If the recombination frequency between genes (A) and (B) is 5.3%, what is the distance between the genes in map units on the linkage map?
Why are pteridophytes better adapted to dry land than bryophytes? Were pteridophytes always less abundant than phanerogamic plants?
What are some of the methods that scientists have used to study the components, processes, and outcomes of cell division?
Assume you have isolated a new recessive tryptophan-requiring mutation in a MAT? yeast strain. You cross this mutant to a MAT? trp1 trp3 strain.
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