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I use an affinity column to purify a protein, "X" from cell lysate. The specific activity of the protein is very low after this step. So I run the affinity-purified sample through an anion exchanger, and collect the early fraction. This eluate has very high specific activity. My results from a PAGE and Western blot after each step are shown. Also shown are the enzyme kinetics after the affinity purification, and after the anion exchange column. Based on all these data, what is your BEST explanation?
Why would this have occurred on the Galapagos Islands, not just to the finches, but to most of the other species found on the island? Be specific--this question is about the Galapagos islands specificall
Compute the number of lipid molecules we added to each cell to make the tethered membrane.
Most transposable elements are flanked by short direct repeats. How are these direct repeats formed?
The allele for unattached earlobes is dominant over the allele for attached earlobes. In a population of 500 individuals, 25% show the recessive phenotype. How many individuals would you expect to be homozygous dominant and heterozygous for this trai..
Compare and contrast a "character" versus a "variant (or trait)".A pea plant with a Tt genotype is crossed to a plant with a tt genotype. What percentage of the offspring do you expect will be dwarf? What is a transgenic organism? Give one example.Wh..
Describe each component of energy expenditure. What factors influence each? How can energy expenditure be estimated?
In a population of chicken, three color morphs defined by two alleles occur: dark brown with genotype BB, light brown with genotype Bb, and white with genotype bb.
Which is true about a transamination reaction
What will be the most likely phenotypic and genotypic ratios when a colorblind woman marries a normally visioned man?
Ajax is about to embark on a project involving the design, manufacture and introduction of a new microwave oven. The first three activities and the immediate predecessors are:
q1. the general public does not always use the terms hypothesis and theory in the similar manner that biologists do.
What is a/the "normal flora" of the human colon? Give a few examples of the bacteria found there.
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