### Polymer diffusion-calibration of an optical tweezer

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##### Reference no: EM135011

Question 1: Polymer diffusion: The figure below indicates the result of a particle tracking experiment where lateral positions of fluorescent polystyrene sulphonate (PSS) molecules were recorded. The curve labels on the graph show the polymer concentration in water μg/ml.

If the viscosity of the pure water is 1x10-3 Pa.s and the measurement is done at room temperature, obtain an estimate of:

a) Size of the molecule (hydrodynamic radius);

b) Degree of polymerisation (assuming the Kuhn length for PSS is 3 °A).

c) Viscosity of the concentrated solutions as a function of concentration. Question 2: Calibration of an optical tweezer: You performed the subsequent calibration experiments at room temp, 25oC for an optically trapped latex bead that is free in solution: x displacements of the trapped bead, in nm, were gathered for some time in stationary water. Fluctuations in position are due to random bombardment of water molecules such as those shown in the figure below (black curve). In the second stage of the experiment, the fluid chamber surrounding the bead was moved along the x direction at a constant velocity of 5.0 μm/ms (red curve). Data from an actual experiment, calib flow.dat and calib noflow.dat, can be found on the Black-board/Assignments page. Modeling the optical trap as a Hookean spring, evaluate the trap stiffness using the Boltzmann distribution. Determine the sphere diameter by comparing the Stokes drag and the tweezer force that balances the drag. Suppose the viscosity of water 10-3 Pa.s. ### Write a Review

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