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You are testing the rate of glucose transport into vesicles using the Na+-glucose pump.
A. In experiment 1, you employ liposomes that have the pump in the same orientation as that found in the plasma membrane in epithelial cells. These liposomes contain glucose but no Na+ ions. You then transfer the liposomes to a series of tubes with solutions containing the same glucose concentration as that inside the vesicle and 0, 1, 2, 3, or 10 mM Na+. You measure the initial rates of glucose transport and plot your results (Figure below). Why do the initial rates of glucose transport into the liposome reach a plateau as the concentration of Na+ increases?
B. In experiment 2, there is one new variable: you have included leaky Na+ channels in the liposomal membrane. You've plotted your results on the same graph as experiment 1 to compare. Explain the reason for obtaining such different results in experiment 2 from those in experiment 1.
Design an experiment to demonstrate that DNA replication is semi-conservative. List two markers that one can use to label the DNA.
In interrupted mating experiments using an Hfr strain in E. coli, you find that two genes, bio and arg, are transferred at about the same time. A third gene, pab is transferred slightly later.
1. Who is Bob Inglis and what happened to him? 2. How have conservative political groups used disinformation and money to sway the opinion of the public and of politicians? 3. Who are the Koch brothers and Donors Trust?
Explain the two types of population growth. Analyze the type of growth exhibited by the human population and its effect on the environment.
Describe one growth factor and one mitogen and the cellular signaling they trigger within the cell, as well as the final result of that signaling. What are carcinogens and why and how might they effect the cell cycle in the transformation of normal ..
Report on Gene and Related Proteinand Determine chromosome location of your gene. Paste a picture of the chromosome from MAP VIEWER into your report and describe
Assume you are a male. Your father was a hemophiliac and your mother had normal blood clotting and was not a carrier for thehemophilia trait. What does this make your genotype for this trait?
You identify a novel bacterial species from a deep sea hyrothermal vent that lacks 5'-dNTPs. The DNA in this organism has the normal anti-parallel double stranded helix with 3'- 5' phosphodiester bonds.
what are the genotypes of a male yellow rat crossed with a gray female rat producing forty-six gray and fifty- three yellow offspring? (Hint: There are three colours for rats; yellow, gray black. The yellow and black are homozygous.)
1. Consider a cross of two heterozygous plants TtGg x TtGg. Use the product rule to predict the probability of TTgg offspring.....What is the probability of TTgg offspring?
individuals that inherit a single inactive retinoblastoma rb allele from one of their parents have a 90 chance of
How would you determine the termination codon appear in the mRNA for this gene? Write the sequence with the 5' and 3' ends of the sequence indicate clearly.
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