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How many copies of a protein need to be present in a cell in order for it to be visible as a band on an SDS gel? Assume that you can load 100 μg of cell extract onto a gel and that you can detect 10 ng in a single band in the gel. The concentration of protein in cells is about 200 mg/mL, and a typical mammalian cell has a volume of about 1000 μm3 and a typical bacterium a volume of about 1 μm3. Given these parameters, calculate the number of copies of a 120-kd protein that would need to be present in a mammalian cell and in a bacterium in order to give a detectable band on a gel. You might try an order-of-magnitude guess before you make the calculations.
apply the alara concept for worker protection during replacement of a pump in a system where removable contamination
Draw a punnet suquare and predict the expedcted phenotype ratio for the following 3 cross and be sure to label the ratios A) ttyy cross TtYY b) TTYy cross TtYy c) Ttyy cross TtYY.
Two (2) liters of a hypotonic 0.5% NaCl solution is infused into the extracellular fluid compartment of a 40 Kg dog with initial plasma osmolarity = 300 milliosmoles/liter.
"A case study is a narrative used to help you practice real-life analysis and communication skills. It is a learning tool that provides readers with "enough detail...to understand the nature and scope of the problem, and...serve as a springboard f..
How do i sequence a peptide using treatment reagents for example the amino acid sequence is ARG,ASP,CYS,GLU,LEU,SER,THR. 1st use- Staphylococcal protease then chymotrypsin then edman's reagent and 2 nitro 5 thiocyanobenzoate. the final sequence yield..
How long will it take for the blood level to reach the non-toxic range?
Noah is a technician assigned to photograph chromosomes. At what stage of the cell cycle are the chromosomes sufficiently condensed that they FIRST become visible under light microscope.
The proportion of adenine bases in the human genome is 27%. What is the probability of observing the six-nucleotide sequence TAGTTA from a random point in the human genome?
The other argues that the phases are caused by the orientation of Earth, the Moon, and the Sun. Explain how the photos in the chapter-opening illustration falsify one of these hypotheses.
Suppose that H,I,J,K, and L represent alleles for the autosomal gene on different chromosomes. if a mating occurs between the individuals with the given genotypes: HHIiJjKKLI X HhIiJJkkLl
A couple are both tested and found to be carriers of the cystic fibrosis gene. If they have 2 children, what is the chance that both will be affected by cystic fibrosis?
Critically discuss three of the test systems used to study the effects of a toxicant on chromosomes, describe each test system, and discuss one advantage or disadvantage of that particular test system.
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