Optical microscopy and scanning electron microscopy

Reference no: EM13249857

1. Compare and explain the reason(s) for the difference in image magnification levels between optical (light) microscopy and scanning electron microscopy.

2. What is meant by “sample charging”, with electron microscopes and why is it an issue? How can it be prevented.

3. When using a scanning electron microscope (SEM), what are secondary electrons primarily use for, and why? What are back-scattered electrons used for, and what are x-rays used for?

4. Briefly describe two methods of biological sample preparation for SEM analysis. Comment on the pros and cons of the methods you have chosen.

5. Describe what is meant by “characteristic x-rays”, and list a few examples where an incorrect elemental composition might be obtained by using these x-rays.

Reference no: EM13249857

Questions Cloud

Compute the amount of heat evolved in the oxidation : Calculate the amount of heat evolved in the oxidation of 32.0 g of Fe (Ar = 55.85 g/mol) at 25 oC and 1 atm pressure ( ?Hfo(Fe2O 3,s) = -824kJ/mol)

Determine the optimal number : Determine the optimal number (0, 1, 2, 3, 4, 5, 6) of rooms that the Zigus hotel should overbook in order to minimize average daily costs.

Determine circuit power factor and draw impedence phasor : A coil is placed in series with a resistance of 30 ohms. When the combination is connected to a 220 V, 60 Hz source, the current is 4.2 A and the power drawn by the circuit is 670 W.

Find the direction of the minimum magnetic field : A wire has a mass per unit length of 0.500 g/cm. what is the magnitude and direction of the minimum magnetic field

Optical microscopy and scanning electron microscopy : Compare and explain the reason(s) for the difference in image magnification levels between optical (light) microscopy and scanning electron microscopy. What is meant by “sample charging”, with electron microscopes and why is it an issue? How can it ..

Determine coil and resistor voltage and overall power factor : A coil with an impedence of 64.1 angle 51.4 degrees is connected in series with a resistance 22.5 ohms. The combination is supplied by 120 V source. Determine the coil and resistor voltages and the overall power factor.

Draft a mission statement for willowbrook school : Draft a mission statement for Willowbrook School, based on information provided in the first two chapters and does a strong business case exist in the case of Willowbrook School? Discuss why or why not.

What advancement in operations management in other countries : Why has it become so important for companies to become competitive in the global business environment?

Find the change in momentum of the ball : a falling rubber ball bounces off the floor. the velocity just before it hits the floor is Vi= (2.7m/2) x + (5.2m/s)y. what is the change in momentum of the ball from just before to just after it is in contact with the floor

User Account

All Pages