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You have 2 tubes containing cultures of genetically distinct bacterial strains. You know that when you mix the two together, you get Horizontal gene transfer (HGT), but you do not know what genetic exchange mechanism is accomplishing this transfer. Prior to mixing the strains, you add an enzyme that degrades any DNA it comes into contact with the tubes and allow it to incubate briefly. You then mix contents of both tubes together and find that you are no longer able to achieve HGT. Both cultures are still completely viable. Name the 3 methods of HGT that we discussed in class. Which of these is the MOST likely process you interfered with? In your answer, explain your observations and why the other two processes are not likely to be involved.
In a separate experiment CC dd individuals were crossed to cc DD individuals. The F1 generation was then testcrossed to cc dd individuals. What would be the result of this testcross.
Explain an experiment that helped elucidate how dna is synthesized-in terms of identifying a protein important for the process.
Lysozyme was assayed at bacterial cell wall, = 2.5x10-8 g. The Km for the substrate = 6x10-3g. At the end of one minute 2.5% of the cell wall hydrolysed.
Which wavelengths of visible light carry the most energy? How does this relate to strategies for plant photosynthesis? Why was it important to mark the TLC plate with pencil?
Explain the differences between eukaryotic and prokaroytic cells. Include in your discussion three phenotypic systems used to classify microbes.
By this time, 35 cells of V. vulnificus have gotten into your blood. However, the doxycycline does slow the growth of the bacteria considerably to a generation time of twelve hours.
You are given a stock solution of EDTA at a concentration of 5mM, how would you dilution of EDTA with a final concentration of 5mM in a final volume of 250mL?
Colonies were then transferred to plates containing a minimum media. To grow on minimum medium, yeast cells must be wild type and able to produced their own amino acids, and nucleotides.
Describe how an increase in the carbon dioxide levels in the blood and interstitial fluid affects the heart, blood vessels, hemoglobin dissociation curve, and the respiratory system.
Think about the two separate experiments. In the first experiment, a DNA strand containing a+ b+ genes were placed in a culture of bacteria with a- b- genes.
what kinds of matings would produce only palominos
As sound intensity increases, we hear the sound as a louder sound at the same pitch.
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