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Your assistant Igor is studying a neurotransmitter-stimulated GPCR pathway, hypothesized to stimulate the expression of genes that increase retention of memory. He plans to test his hypothesis using a cell line expressing this GPCR. If his hypothesis is correct, what would be the expected outcome on memory gene expression? Explain you reasoning for each answer. d. a mutation in the nuclear import sequence of PKA from PPKKKRKV to PPAAAAAV e. deletion of a functional phosphatase for the gene regulatory protein
Four proteins, each having a single polypeptide, are separated by SDS-PAGE: human cytochrome c , human serum albumin, bovine myoglobin.
For each of the E. coli strains given below, estimate the effect of the genotype on expression of the trpE and trpC genes in the presence and absence of tryptophan.
Studies have demonstrate that the effect of neuropeptide Y on smooth muscle cells in the uterus is attenuated doing pregancy.
Assume you leave a culture inthe 37 C incubator with out tryptophan for week instead of overnight like you planed. to your surprise culture has shown some visible growth so you reinoculate from that flask into a new flask
explain the advantage and disadvantage of random addition or deletion of nucleotides at VDJ junctions during re-arrangement of immunoglobulin gene segments.
Vinblastine is a pharmaceutical used to treat several cancers, such as lung and breast cancer and Hodgkin's lymphoma. It binds tubulin, a protein found in microtubules of cytoskeleton
A carnivorous bird (feeding on both herbivore/detritivore and carnivore levels) has an energy requirement of 18 kcal/day. If it feeds in our ecosystem for nine months (270 days) of the year, how large must its territory be to ensure its dietary ne..
Determine the metabolic effects of the increase in epinephrine and norepinephrine in the liver, muscle and adipose tissue at the onset of exercise?
When glucagon binds to it is receptor on a liver cell, which one of the following sequences of events occurs to activate protein kinase A?
Compute the number of "net ATP" molecules obtained the anaerobic conversion of each of the following sugars to lactate.
Suppose that in rats, grey fur (T) is dominant to tan (t). If you mate a tan rat with a homozygous dominant rat, Determine what fraction of the offspring will be homozygous?
If a 100X solution is 1 M, what is the concentration of a 1X solution? Explain how you would prepare 100 mL of a 0.5 percent w/v agarose in 1X TAE buffer starting with a 20X TAE stock solution and solid agarose powder.
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