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A colleague has isolated several mutants that behave normally at low temperature, but at high temperatures exhibit anomalous behavior before or during S phase. You suggest that she characterize the mutant phenotypes at high temperatures in more detail by determining if the pre-replicative complex (pre-RC) forms normally and investigating if the replication origins can fire to initiate DNA synthesis. Once she has finished the suggested experiments, she returns for your help in interpreting the data. Match the phenotype in the left column with a proposed defect in protein function from the right column that may explain each phenotype.A. Cells arrest in G1with normal pre-RCB. Cells arrest in G1with no pre-RCC. Origins fire more than once during a single S phaseD. Cells replicate DNA normally but cannot proceed to M phase1. S cyclins cannot be degraded2. Cdc6 cannot bind ORC3. S cyclins cannot bind Cdks4. Cdc6 cannot be ubiquitinated
Can viruses evolve? Why or why not? a. No, only their hosts can evolve. b. Yes, their genetic make-up responds to natural selection. c. No, they are not alive. d. Yes, they are able to alter their genetic code directly in response to selection pre..
A genomic library is made for a haploid organis with a genome of 3 X 10^9 bp. If you want to clone five genome equivalent into a vector library.
Detail the hormones responsible for maintaining fluid and electrolyte balance. Critically discuss the three primary buffering systems.
5-different mutations were derived from base pair substitutions at a single codon. In this codon, the mutant alleles had arginine, leucine.
The pUC series plasmid contains LacZ gene with multiple cloning site. Suppose if you insert a piece of DNA into this LacZ gene, how will you tell which one is your recombinant.
Provide list of Health Insurance Portability and Accountability Act requirements such as transaction and code set standards, privacy standards?
In mice, black eyes (B) are dominant over red eyes (b) and short ears (S) are dominant over having a long floppy ears (s). A black eyed, short eared mouse is crossed with a red eyed, long-eared mouse. The offspring has black eyes and long ears.
Two closely related species of wolf spiders live together in northern California. However, meadow wolf spiders are only observed in meadows and forest wolf spiders are only observed in the forest. Both species are found near forest-meadow edges.
What is a molecular diagnostic method that could be used to help identify the cause of and unknown infection.
Mary is in an automobile accident and injures her spinal cord. She has lost feeling in her right hand and her doctor tells her it is the result of swelling compressing a portion of her spinal cord. Which part of her cord is possible to be compressed.
A locomotive train is on its way from Chicago, IL to Madison, WI. The trip is said to last 3.15 hours. When train arrives in Madison the conductor notices it actually took them 3.26 hours.
Acetylcholine binds this membrane protein, which subsequently opens to allow sodium to enter the cell. Acetylcholine exerts its effect on what kind of transport protein.
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