Map a Drosophila gene in your own time

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Reference no: EM131964909

PROJECT - Mapping an unknown Drosophila gene

The aim of this exercise is to allow you the freedom to map a Drosophila gene in your own time and using your initiative. You are assigned an autosomal recessive mutation to map to its two closest markers based on provided dihybrid test cross data. Only map your gene with respect to the other autosomal genes shown in Table 1. Note that you cannot analyse the results of crosses between two different genes that affect the same body part as these genes will interact.

Scenario: You are an honours student and your project is to characterise a homozygous recessive mutant strain of Drosophila. A previous student has generated dihybrid testcross data for 10 other autosomal recessive genes from Drosophila and this data is available to you (on Moodle) to map your assigned gene to its two closest markers. Work with the provided data and then compare your findings with published recombination distances to determine the accuracy of your map. Discuss if the data that has been provided to you is sufficient to produce an accurate map in comparison to published data, and suggest future improvements that will make your map more accurate. Also discuss both the function of your assigned gene and the broader utility of gene mapping, for example for human disease genes.

Table 1: Recessive mutations in FlyLab and the body part they affect

Body colour	Eye colour	Bristles	Wing size	Wing shape	Wing vein
Black	Brown	Forked	Apterous	Dumpy	Crossveinless
Ebony	Purple	Singed	Miniature	Scalloped	Radius Incompletus*
Sable	Sepia	Spineless	Vestigial
Tan	White
Yellow

*This mutation was later renamed Knirps - the gene is now known as this

Report structure (word limits are a guide only).

Abstract (word limit 150 words) - Abstracts provide a summary of the project. They should start with some brief background information (this should include a sentence giving a broad introduction to the field, then a sentence of more detailed background relevant to your study). This should be followed by the results, and then the main conclusions.

Introduction (word limit 500 words) - This should introduce and explain the concepts behind genetic mapping and how maps are made. Do not copy the lecture notes or the prac manual or cite these. Cite where appropriate (eg, general reviews).

Aims - This should be brief and specific.

Results - Include here the mapping results for the two closest genes to your assigned gene and a comparison with published data on map distances that you have found.

Discussion (word limit 1000 words) - Briefly summarise the results that you obtained and discuss any discrepancies that you see between your data and published sources on map distances. Discuss how the experimental approach could be improved if given an opportunity to set up your own crosses and generate your own data.

Include in your discussion the function of the gene that you have been assigned. Comment more widely on the importance and significance of gene mapping.

References - Only list the references that you cite in the text. Please use the CSIRO referencing style. The CSIRO citing and referencing guide is available on Moodle.

Appendix - Present here additional cross data not included in the main results section (with justification) making sure that the main report refers to the appendix.

Attachment:- Assignment Files.rar

Reference no: EM131964909

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Reviews

len1964909

5/1/2018 3:55:57 AM

Your report will be marked based on the mapping project rubric available on Moodle. Turnitin similarity percentages should not be above 20%. However, if the overlap is simply due to the use of common genetic phrases or mapping data, please do not be overly concerned as we will look at reports with high Turnitin scores to detect these common phrases and you will not be penalised. Please submit your mapping project in hardcopy format (with Turnitin report). Please use the latest assignment cover page for submission. You can submit the hardcopy of your report into the assigned pigeon hole located at Building 4, Level 8.

len1964909

5/1/2018 3:55:51 AM

Formatting: Font should be one of 12pt Times New Roman, 11pt Arial or 11pt Calibri, 1.5 spacing , Word limits are as suggested to the Abstract, Introduction and Discussion sections. Please indicate the number of words in these sections in your report. Other sections have no word limits but the report should be no more than 10 pages long, excluding appendices. Penalties will apply for reports which are above the page limit.

len1964909

5/1/2018 3:55:44 AM

Clearly diagram crosses including genotypes not just phenotypes. A written report of this project is due by 10 am on Wednesday 2nd (week 9) and counts for 10% of your final GEN2041 mark. If you are unable to have your report in on time for what you feel are exceptional reasons, you must submit a completed special consideration request form and evidence before the deadline into the Mapping Project Special consideration drop box. Please note that 10% of the total potential mark will be deducted from late reports for each working day the report is late unless an extension has been obtained before the deadline. In addition the report cannot be accepted more than one week after the due date except in exceptional circumstances and in consultation with the coordinator.

len1964909

5/1/2018 3:55:39 AM

Main purpose and idea of research described well. Analysis and interpretation all correct and well presented (4 marks) Two closest markers identified by calculating recombination distances and their order. Appropriate data presented in results section and whole report is well connected. Only data relevant to the gene and it's closest markers are presented in the results. Rest of data to be refered to the appendix. Good and detailed discussion of comparison to published data (4 marks).

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