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Isolated a set of conditional mutants

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Reference no: EM132016979

Supposing you have isolated a set of conditional mutants in each enzyme of the pathway.

Call them 1, 2, 3, 4, 5, 6 and 7. The following feeding table is observed, in which growth is observed if the compound is added:

Mutant

PEP

3PG

G3P

F1,6DP

1

+

-

-

-

2

+

-

-

-

3

+

+

-

-

4

+

+

-

-

5

+

+

+

-

6

+

+

+

+

7

+

+

+

+

Order the mutants using these data  and specify a possible enzyme that is temperature sensitive for each.

Reference no: EM132016979

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