Initial enzyme concentration

Reference no: EM13333831

An enzyme ATPase has a molecular weight of 5 x 104 daltons, a KM value of 10-4M, and a k2 value of k2 = 104 molecules ATP/min molecule enzyme at 37°e. The reaction catalyzed is the

following:

ATP

ATPase) ADP+Pj

which can also be represented as

E+S--hES~E+P

.......----k,

where S is ATP. The enzyme at this temperature is unstable. The enzyme inactivation kinetics are first order:

E =Eo e^(-k,r)

where Eo is the initial enzyme concentration and kd = 0.1 min-I. In an experiment with a partially pure enzyme preparation. lO)..lg of total crude protein (containing enzyme) is added to a 1 rnl reaction mixture containing 0.02 M ATP and incubated at 37°C. After 12 hours the reaction ends (i.e., t --t 00) and the inorganic phosphate (Pi) concentration is found to be 0.002 M, which was initially zero. What fraction of the crude protein preparation was the enzyme? Hint: Since [S] » Kin' the reaction rate can be represented by

d( P)/dt = k 2 [E]

Reference no: EM13333831

Questions Cloud

Declared the array of integers within the main function : Write a program that returns the min, max and mean value of an array of integers named data ar. Your program should call a single function that returns that mean, max and mean value of the array.

Draw a network flow model to represent the problem : A cotton grower in south Georgia produces cotton on farms in Statesboro and Brooklet, ships it to cotton gins in Claxton and Millen, where it is processed, and then sends it to distribution centers in Savannah, Perry, and Valdosta, where it is sold t..

Explain effect of relationships among television-movies : Explain the effect of relationships among television, movies, and electronic games with culture. Include the following: How culture is reflected in movies and television?

What exactly is 3rd grade state citizenship : What exactly is 3rd Grade state citizenship--i mean i have an idea- but what else can i use to write a lesson plan. so far, i have come with , how to be a good citizen, voting, what it means to be a citizen as opposed to being a non citizen, etc.

Initial enzyme concentration : An enzyme ATPase has a molecular weight of 5 x 104 daltons, a KM value of 10-4M, and a k2 value of k2 = 104 molecules ATP/min molecule enzyme at 37°e.

Argument is based on a false dilemma : Specifically, is Rand correct in saying that selfishness is a virtue and that altruism (helping others) is not a moral good that we should pursue? What about Pojman's criticism of Rand's view? Is he correct in saying that her argument is based on a f..

Differences between terrestrial and jovian planets : Which are the terrestrial planets? Why are they called "terrestrial? Which are the Jovian planets? Why are they given that name? Name three important differences between terrestrial and Jovian planets.

How do i find companies of same trade : Select four publicly traded companies: one that would be in the same industry as Riordan Manufacturing, one in the same industry as Huffman Trucking, one in the same industry as Kudler Fine Foods and one in the same industry as McBride Financial Serv..

How did for-profit organizations affect the science of fligh : When did aviation become an industry? How did for-profit organizations affect the science of flight?

User Account

All Pages