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Question: Shigella flexneri is an intracellular pathogen closely related to E. coli and S. typhimurium. Some experiments have shown that oxygen controls expression of some virulence factors in this organism. Assume that you have a S. flexneri gene, oxyA, that appears to code for a virulence factor and is only expressed in the absence of oxygen. Design a set of experiments to: 1) identify additional genes that are also expressed in the absence of oxygen but not in the presence of oxygen and 2) determine if expression of the oxyA gene in the absence of oxygen is due to the action of a repressor or an activator protein. Be sure to describe any strains or clones you would need to create to carry out your experiments and the kind of results you would expect from each experiment.
What techniques might be used to characterize the structure and composition of the structure. Which of the follow will the nurse include in her teaching plan for this patient.
In order for the 4-nitrophenol to release the yellow colour expected, it has to be fully ionised. With a pKa of 7, I have decided that pH9 will allow it to be fully ionised - this is also supported by yellow colour given off, and a reasonable calib..
Chose to do an informational interview, start thinking of your topic and interview questions now. See the course project description for more information on this project component.
Determining Diffusivity of Solutes in a Liquid Compute the diffusion coefficient of a protein macromolecule bovine scrum albumin (BSA) at 23degreeC.
What kind of approach is this alien taking to learn about the car? An alien from a new planet landed on earth. He is attentive by cars and is determined to figure out how they work.
Discuss and explain the role of Maternal Affect Genes, Gap Genes, and Pair-rule genes in the anterior-posterior patterning of the Drosophila embryo.
Compared to a carbon atom with only single covalent bonds, a carbon with a double bond 1 Shares fewer electron pairs with other atoms two Is more flexible in the spatial arrangement.
Fruit flies have 4-main chromosomes. Fruit flies also have number of body markers that are useful for mapping genes to given chromosomes.
Presently completed a small chart on comparison of potential deficits among nations given United States style of water consumption.
why the average person secretes about 2.5 litres. where C2 is the greater concentration. If the process of pumping H+ ions is 20% efficient, how many moles of ATP are used each day to secrete gastric juice? How many kilograms could be lifted one me..
The DNA molecule is permitted to replicate in a solution containing all four unlabeled deoxyribonucleoside tripohosphates and deoxyadenosine triphospage that is labeled with 32P.
A zookeeper hypothesizes that converting the intensity of the light in the primate exhibits will reduce the amount of aggression between the baboons.
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