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The hydrolysis of pyrophosphate to orthophosphate is important in driving forward biosynthetic reactions, such as the synthesis of DNA. This hydrolytic reaction is catalyzed in E. coli by a pyrophosphate that has a mass of 120 kd and consists of six identical subunits. For this enzyme, a unit of activity is defined as the amount of enzyme that hydrolyzes 10 umol of pyrophosphate in 15 min at 37 degrees C under standard assay conditions. The purified enzyme has a Vmax of 2800 units per milligram of enzyme.
a) How many moles of the substrate is hydrolyzed per second per milligram of enzyme when the substrate concentration is much greater than Km? (Draw a velocity versus substrate concentration graph to show your point)
b) How many moles of active sites is there in 1 mg of enzyme? Assume that each subunit has one active site. c) What is the turnover number of the enzyme? (Don't bother comparing it to other enzymes; just show all your own, which equations you use, what numbers you use, where you get them from, etc.)
A black guinea pig crossed with an albino guinea pig produce 12 black offspring. While the albino was crossed with a second black animal, 6 blacks and 6 albinos were obtained. What is the best justification for this genetic situation.
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Substrate-level phosphorylation occurs within the metabolic pathway where sufficient energy is released by given chemical reaction to drive the synthesis of ATP from ADP and phosphate. In which the metabolic pathways are substrate-level phosphorylati..
define the correct sequence of events in the synthesis.
Elucidate where they came from with an easy diagram. The 10 with normal wings also have mutant green eyes and mutant hairy legs.
Two consecutive crosses, with the first between pure-breeding parents that differ in two phenotypes to create an F1 generation, and the second in two F1 progeny to create an F2 generation.
The genes dumpy wings, clot eyes, and apterous wings are linked on chromosome II of Drosophila. In a series of two-point mapping crosses, the genetic distances demonstrate below were determined.
plan the structure of butanol and show water molecules in hydrogen bonds to the butanol. How many should there be? remember the H bonding rules.
Describe how the hypothalamus controls secretion from the pituitary gland. Be specific regarding circulation and communication between the two regions.
Biotechnology permits the use of living organisms or their processes for human needs or purposes. Currently, this topic includes such general examples as cloning,
Describe why it may matter if the county had dry areas, swampy areas, forests, and meadows all within its boundaries. As a doctor, which of the statements are important factors for you to consider as you decide whether or not to remove the patient's ..
Discuss the defining characteristics and methods of reproduction of organisms belonging to the Kingdom Fungi in general,
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