Hybridization solution containing the working concentration

Assignment Help Biology
Reference no: EM132280293

SSC buffer is frequently used as an ingredient in DNA hybridization studies. You have 550 ml of a stock solution of 0.2 M SSC buffer. You need to make 100 ml of hybridization solution containing the working concentration of 50 mM SSC buffer. How much SSC stock should you have to use to make this new solution? Round to the nearest hundredth. In mL

Reference no: EM132280293

Questions Cloud

What is the concentration of sucrose solution : You have weighed out 5.85g of sucrose and dissolved it in a final volume of 200ml of water.
Analyze the steps in the budgeting process : Analyze the steps in the budgeting process summarized in the NASBO presentation and discuss how the actions in the process can improve the value and reliability
Discuss about the imprisoning communities : During the first three chapters of Imprisoning Communities, Dr. Clear argues that the imprisonment policies currently used in the U.S. do not work.
Enzyme reaction cocktail that contains : You wish to make a 30 µl enzyme reaction cocktail that contains 100 µM MgCl2. The concentration of your MgCl2 stock solution is 0.8 mM.
Hybridization solution containing the working concentration : You have 550 ml of a stock solution of 0.2 M SSC buffer. You need to make 100 ml of hybridization solution containing
Final concentration of dntps in the reaction cocktail : What is the final concentration of dNTPs in the reaction cocktail? Round to the nearest hundredth.
What total amount should be credited : What total amount should be credited to additional paid-in capital from common stock as a result of the conversion of the preferred stock into common stock
What should be Crane earnings per common share : Net income for the year ended December 31, 2018, was $954000. What should be Crane's 2018 earnings per common share, rounded to the nearest penny
Describe what the oftball coaches have done in an effort : Describe what the a) AD, b) the softball coaches, and c) the umpires should have done in an effort to avoid the injury that occurred in this instance.

Reviews

Write a Review

Biology Questions & Answers

  Develop a vaccine for a certain type of flu bacteria

Bacterial mutations are a great example of natural selection, especially those that cause the flu. But what happens when we can't develop a vaccine for a certain type of flu bacteria? If it turns out to be a deadly type, think about the population..

  How is specific trna recognition and binding accomplished

Considering that binding of the correct tRNA to the aminoacyl-tRNA synthetase is the critical first step (amino acid proofreading occurs after tRNA binding)

  Definitions of ecological footprint and ecological overshoot

In your own words, what are the definitions of ecological footprint and ecological overshoot?

  Why prokaryotic cell probably appeare before eukaryotic cell

Discuss why prokaryotic cells probably appeared before eukaryotic cells. Hypothesize whether prokaryotic cells might have been symbiotic before the evolution of the eukaryotic cells.

  Why would you want to get an amino acid composition first

Amino acid compositions can be determined by heating a protein in 6 M HCL and running the hydrolysate through an ion-exhange column. If you were going to do an amino acid sequencing experiment, why would you want to get an amino acid composition f..

  Agriculture applications and specific examples

Explain the use of the Ti plasma in agriculture applications and specific examples.

  Smooth muscle and striated muscles

Smooth muscle and striated muscles forms of distinct mRNA because .....A. aminoacylationB. polyadenylation

  Identical age-specific schedules of birth and death rates

Two populations of elk have exactly the same total population sizes and identical age-specific schedules of birth and death rates, and yet these two populations are currently growing at different rates. How can this be?

  List the three common side effects of inhaled steroids

The patient was discharged from the hospital 10 days later. As the discharging nurse, list three medical issues you would advise this patient to attend to. List the issues in the order of importance: from most to least.

  What is osteoporosis

What is osteoporosis? Common Signs and symptoms seen Screening assessment tools

  Vectors to show overall direction of replication

DNA strands (parental, leading, lagging, 2 or more Okazaki fragments), polarities (5' or 3'), all replication enzymes, binding proteins, primers

  Rhetorical analysis on a public document

How do you write a rhetorical analysis on a public document with out is sounding like a expository essay?

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd