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Alcohol dehydrogenase (AD) is an enzyme which catalyzes the reaction of its natural substrate ethanol to form acetaldehyde.The Km of AD, from rhinoceros livers, for ethanol is 1 X 10-3M.
This enzyme is however somewhat non-specific and will recognize substrates other than ethanol. How would the kinetic plot be affected if AD were to separately catalyze methanol and isopropanol instead of ethanol?Assume that the overall Vmax remains the same in all 3 cases.
How would the Km change for methanol compared to ethanol (higher, lower, the same)?
How would the Km change for isopropanol compared to ethanol (higher, lower, the same)?
How would the Km's of methanol and isopropanol compare (which higher than the other or about the same).Based upon your knowledge of the mechanisms by which enzymes work, briefly explain how you decided to place your new Km's.
How many times would you expect a restriction enzyme that recognizes a 6 base palindrome to cut a 16500 base piece of of DNA?
Low levels of carnitine can cause muscular weakness during exercise. Why, and will it affect metabolism of glycogen?
The sister of a great-grandfather shared by first cousins died of an autosomal recessive disorder. If these cousins mate, what is the chance that their child will have the disease?
The external environment is open directly to the peritoneal cavity of the abdomen and pelvis. This is one source of pelvic inflammatory disease.
Compare and contrast the benefits and consequences of salt and sugar as a preservative. How could a food manufacture that produce food intended for a low salt diet ensure a long shelf life?
Suppose you are taking a cruise from California to Hawaii. About halfway there, the ship begins to sink. You are able to board a lifeboat, but now you are floating in the ocean waiting to be rescued.
On a normal ordered chromsome, two loci, a and b, lie 15 map units apart on the left arm of a metacentric chromosome. A third locus, c, lies 10 map units to the right of b on the right arm of the chromosome.
So I know that the oocyte and the sperm are the female and male"germ cell", and that the germ cells give rise to gametes, whichfuse at fertilization. What's the process of a germ cell becomingactual "gametes"? (especially in females) Is it a matte..
Describe three processes that are non adaptive at the organismal level, but that play an important role in the eolution of the structure and content of eukaryotic genomes.
Actions of the biceps brachii muscle include: supination of the forearm, Flexion of the shoulder, Flexion of the elbow.
After some time, you start your experiment. Before exposing the population to pesticide, you are able to determine that the frequency of the resistance allele in the sample is 0.08.
All enterics are facultative anaerobes; that is, they have both respiratory and fermentative enzymes. What color results would you expect for organisms in O-F glucose media inoculated with an enteric? Remember to describe both sealed and unsealed ..
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