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We are supposed to draw a lineweaver burke plot to calculate the Km and Vmax, which I am able to do, except the units for the substrate are supposed to be micromolar, which I have, but the reaction velocity is supposed to be sketched in micromolar/minute and we are only given optical density/minute. How to you convert Optical density/minute to micromolar/minute based on the given information?? Substrate Concentration: (micromolar) 0 2.31 4.61 9.23 18.45 36.90 55.35 73.80 92.23 Optical Density/ Minute (Reaction velocity) 0 .2 .37 .55 .89 1.32 1.4 1.46 1.52 Product Standard Concentration Curve: Product Concentration (micromolar) 0 1.42 2.84 5.67 11.34 17.01 Absorbance at 405nm 0 .09 .21 .39 .81 1.19
As a laboratory assistant you were asked to determine the total number of bacteria in the urine of a person with a urinary tract disorder.
Compute the frequencies of the AA, Aa, and aa genotypes after one generation if the initial population consists of 0.2 AA, 0.6 Aa, and 0.2 aa genotypes
as calcium and magnesium salts of fatty acids are insoluble in water, how would you expect this to affect the usefulness of soap in hard water.Why would these results happen the way they did.
G for complete oxidation of glucose is -686 Kcal/Mole. According the G for ATP and number of ATP's produced from the complete oxidation of glucose calculate the efficiency of biological oxidation of glucose by respiration.
Illustrate one experiment that shows that shh is the critical molecule produced by the inducing tissue referred to in (a). what is the evidence that this tissue regulates the ventral pattern of the neural tube (even before this tissue was known to pr..
Enzymes are typically required to catalyze reactions that would be very unlikely to occur on their own. Suppose that the activation energy, Ea, for the uncatalyzed cheese reaction at 20C is 18kcal/mol.
Hydrolases are one important class of enzyme that function to catalyze.
You are designing a new single-celled organism. Discuss the problems of size, getting molecules like nutrients and wastes in and out, temperature, energy and replication of genetic materials.
Think about the trp operon. For each condition below, indicate whether the cell will synthesize the enzymes coded by trpE, trpD, trpC, trpB, and trpA and be able to synthesize trypophan.
Imagine that non disjunction occurred throughout the production of the egg cell that produced your dragon's baby and resulted in no sex chromosome in the sperm cell. How would this change phenotype of your dragons' baby? Be exact for each trait that ..
Explain chromosomal mapping, what is it? Mapping recombination, linkage maps and physical maps?
Read over the description of the Human Inherited Traits in the Week 6 Experiment instructions in our course and complete the table given below.
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