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1. How can we make an antibody to a specific protein?2. In what ways can this antibody be used as a molecular tool in studying this protein?3. Explain the difference between an allosteric and competitive inhibitor.4. What are the four levels of protein organization? Explain them.
5. In SDS-Page, proteins are separated by length. If the same length proteins can fold into loose (bigger) or condensed (smaller) shapes and can even complex with other proteins, how are individual proteins separated solely according to their length? Additionally, proteins can have different charges. Proteins with more basic amino acids have a greater negative charge while proteins with more acidic amino acids have a greater positive charge. Proteins travel from the negative electrode to positive electrode in SDS-Page. How does their intrinsic charge not affect their migration (true for most proteins)?
What percent of all the enzyme molecules will have their Cys thiol in the active form at pH 8.6. Some mammals might run or fight when frightened. For both of these examples, illustrate the physiological mechanisms involved in the answer.
For double helix formation, ?G can be measured to be -54 kJ/mol at pH 7.0 in 1 M at 25 degrees C. The heat released indicates an enthalpy change of -251 kJ/mol (-60 kcal/mol)
What would be the effect of the same analogue on signal transduction of an epithelial cell after exposure to epidermal growth factor (EGF)? How would this compare to the effects of the cholera toxin on these same cells.
Suppose the diameer of a neutral helium atom is 1.40 x 10 to the second power pm. supposed that we could line up helium atoms side by side in contact with one another. How numerous atoms would it take to make the distance from end to end 9.00cm.
a series of protein free patient blood samples at time; 0, 30, 60, 90, 120, 180 mins. I also have a standard sample. I have measured absorbance of these samples; 0.655, 0.717, 0.689, 0.430, 0.852, 0.477 respectively
Find out the number of triplet codons feasible
Phosphonylation, a chemical surface treatment, has been proposed as a means of increasing the hydrophilicity of LDPE, and therefore increase its lubricity and reduce vascular tissue damage.
The physical process through which water and solute move through a membrane when hydrostatic pressure gradient exists across the membrane is the process of?
Would two nucleotides at a time be sufficient to provide enough codons to code for all twenty amino acids? Discuss how many amino acids could be coded for by codons containing only two nucleotides?
Assume you had supply of nucleotides made with atoms that were twice as heavy as normal, and you permitted some cells to replicate their dna once, using only heavy nucleotides.
The presence of which regulatory proteins actually ‘cycles' throughout the cell cycle to help regulate the mechanism of the molecular regulation of the cell cycle clock?
Explain how sexual reproduction in plants illustrates alternation of generations. Include the processes involved and include at least one type of plant to illustrate your answer.
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