How to calculate transformation efficiency

Reference no: EM13121914

Bacteria that have been exposed to two different bacterial strains: one carries only a gene for resistance to the antibiotic Ampicillin; the other carries the gene for Ampicillin resistance as well as a gene for green fluorescent protein (GFP), which glows under UV light. Some bacteria took up the Ampicillin-resistance gene, others took up the genes for Ampicillin resistance and for GFP, but some took up neither (non-transformed). The bacteria were then spread on petri dishes with either nutrients and Ampicillin or just nutrients.

Why do you plate only a small amount of the bacterial solution on the Ampicillin-treated plates, and an even smaller amount on the plates without Ampicillin, when you are trying to calculate transformation efficiency.

Reference no: EM13121914

Questions Cloud

How many apples and hotdogs will mathew buy : Mathew's utility function implies that the marginal utility of apples is 0.5vX/Y and the marginal utility of hotdogs is 0.5vY/X . How many apples and hotdogs will Mathew buy?

Why observed value is not unusual for individual light bulb : Explain why an observed value of 250 hours is not unusual for an individual light bulb, even though it is outside the confidence interval you calculated.

Which structure found in angiosperms : Angiosperms are more advanced than gymnosperms because gymnosperms lack which structure found in angiosperms?

What is an instrumental variable : What is an instrumental variable and Angrist and Krueger use quarter of birth as an instrument for education and explain why quarter of birth may affect education

How to calculate transformation efficiency : Why do you plate only a small amount of the bacterial solution on the Ampicillin-treated plates, and an even smaller amount on the plates without Ampicillin, when you are trying to calculate transformation efficiency.

Test claim for registered drivers from the department : To test this claim, a researcher obtains information about registered drivers from the department of motor vehicles and selects a sample of n=300 accident reports from the police department.

Cost of goods manufacturing during the year : If the cost of goods manufactured during the year amounted to $1,330,000 and annual sales were $1,996,000, how much is the amount of gross profit for the year?

Explain the distinction reimbursement grants : Explain the distinction between reimbursement grants and entitlements. Explain how does this distinction affect the way each type of grant is accounted for?

Sequence of polynomials : Prove that there is a sequence of polynomials p_n on [0,1] such that p_n ----> 0 pointwise on [0,1] but the integral from 0 to 1 of p_n dx

User Account

All Pages