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Hybridoma technology helped to make ELISA techniques available but also provided us with new possibilities for disease treatment. What is the basic process of producing a hybridoma? Your textbook describes Rituximab (Rituxan) and its use in treating B cell lymphomas. Do an online search and describe another currently available monoclonal therapy (see if you have a competitor), including whether the antibody is humanized, chimeric, or some other form; the purpose (target disease) for the monoclonal; and how the monoclonal is intended to treat the disease.
Explain why only certain cell types are permissive for transcription of HIV proviral DNA. What cellular factors are involved and why?
If a particular experiment was known to have transformation efficiency of 3x1063 bacteria/ug of DNA how many transformant colonies would be expected to grow on the LB/amp/ara plate? You can assume that the concentration of DA and fraction of cells..
From an epidemiological perspective, why are chronic and pervasive environmental toxicological exposures so difficult to investigate? What factors make these types of exposures difficult to assess
Glucose is experimentally synthesized with C-14 at carbon 1, and used as a substrate for aerobic metabolism. Where is the label at the end of glycolysis.
A human nerve cell with an average surface area of 250,000 micrometers squared has an extracellular potassium ion concentration of 3mM and an intracellular potassium concentration of 90mM.
A somatic cell of a given plant species contains threebillion base pairs of nuclear DNA and 12 functional centromeres when observed at the conclusion of stage G2 of the cell cycle.
After performing a ligation reaction between the DNA fragments and cleaved vector, you analyze the products of the reaction by gel electrophoresis.
What is meant by energy coupling? Which molecule is most commonly used in energy coupling? Discuss enzyme and catalyst
though , the addition of a trace of uniformly labeled [14C]acetyl-CoA in the presence of an excess of unlabeled malonyl-CoA to a soluble liver fraction yields palmitate labeled with 14C only in C-15 and C-16.
Clarify the effects of the ecb infestation you used. Were all corn varieties evenly effective at controlling the ecb? How do you know.
Assume you are testing a hypothesis that population density of a particular plant species influences the rate at which a pathogenic fungus infects the plant.
In the different case of cytosine deaminating to uracil, how does a repair mechanism determine which base to repair?
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