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1. How much 4X SDS-PAGE sample buffer do you need to add to 10 ul of a cell extract to run it out on an polyacrylamide gel?
2. You wish to plate out 5 x 104 lymphocytes in a total of 2 ml for a focus forming assay. You dilute your cells 1:100 with crystal violet and count them with a hemocytometer. The average cell count is 25. What volume of cells would you add to what volume of medium to prepare your assay? Show your work!
3. An antibody to be used for Western blotting needs to be diluted 1:5,000 for optimum results. What volume of the antibody stock solution do you need to add to 100 ml of buffer to make the blotting solution? By the way, what other components should this buffer solution contain?
4. You have 20 ml of a protein sample in a 10% (w/v) suspension of sodium sulfate. How many rounds of dialysis (in a total volume of 1 liter) would be needed to reduce the salt concentration to 1 mg/ml or less?
Discuss and explain the role of antidiuretic hormone and aldosterone in the maintenance of blood pressure. Fairly detailed please.
What is the M locus, D locus, and Bk locus, in terms of the relationship among genes, alleles and chromosomes.
Reproductive potential is the same for every generation oforganisms. The rawmaterial for evolutionary change are mutations. Microevolution refers to change of microbial populations. Thebiological definition of a species refers to those individuals th..
Compare functional magnetic resonance imaging and magnetoencephalography with regard to spatial and temporal resolution.
Different type of potatoes have varying natural sugar concentrations. explain how this may influence the water potential of each type of potato
Where do histone proteins come from, considering mRNA's are made into proteins outside the nucleus.
During the normalcardiac cycle, the arteria contract when they are directly stimulated by:
What proportion of theresulting progeny will have genotype AB / ab?
A new kind of tulip is produced that develops only purple and pink flowers. Assume that flower color is controlled by a single-gene locus and that the purple allele (C) is dominant to the pink allele (c). A random sample of 1000 tulips froma larg ..
When oxygen is unavailable, some cells use anaerobic(fermentation) pathways to make ATP. Another textbook saysregarding fermentation: "The final steps serve only toregenerate NAD+." What does this statement mean? Explainusing examples of specific fer..
The process by which organisms acquire DNA from different species is most accurately referred to as.
Which is true of the intermediate step of metabolism?
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