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Cystic fibrosis is an autosomal recessive disease in which the cystic fibrosis transmembrane conductance regulator (CFTR) protein is abnormal. The transcribed portion of the cystic fibrosis gene spans about 250,000 base pairs of DNA. The CFTR protein, with 1,480 amino acids, is translated from an mRNA of about 6,500 bases. The most common mutation in this gene results in a protein that is missing a phenylalanine at position 508 (?F508).
1. How many nucleotides are translated in the CFTR protein?
2. With regard to the ?F508 mutation: At the DNA level, what specific alteration has occurred? (3 points)
3. Show the exact change in sequence of #2
Huntington's Disease is a genetic condition inherited as an autosomal dominant and red-green color blindness is an X-linked recessive situation.
Your superb PhD project has earned you a post-doctoral position in a top lab developing puffer fish as a system for studying gene organization. Your goal is to find a puffer fish homologue of a human oncogene (a gene that causes cancer).
Assume if all 3-Bithorax complex genes are deleted, how you expect segments of larva to develop? The segments are written in anterior to posterior order.
What would be TRC for a male with the genotype AABbCcDD? For a female. In contrast, each dominant allele in an individual's genotype contributes 12 ridges to the TRC in males and 10 ridges to the TRC in females.
Life cycle of a fern scenario: Hybrid 1- the sperm in the antheridium has 8 chromosomes; dominant gene 'A' is on one of the chromosomes that produce large sporophyte fronds.
Describe the structure and function of the alveoli. If the muscles used were live, not glycerinated, what would you predict would be the result of adding ATP + Mg to the outside of the muscle cells?
A short-tailed mutant of mouse was discovered. Multiple crosses of this mouse to normal mice produced 27 normal, long-tailed mice and 25 short-tailed mice.
How various ATP equivalents are required to synthesize de novo one molecule of IMP, starting from ribose-5-phosphate? Presume that all required precursors in the pathway are present.
A nine year-old boy decided to find out how long he could breathe into and out of a paper bag. After ~2 minutes his friends noticed that he was breathing very rapidly.
A glycine residue ecists at position 210 of the tryptophan synthetase enzyme of wild-type E.coli. If the codon specifying glycine is GGA, how many single-base substitutions will result in an amino acid at position 210, and what are they? How many ..
A surgical procedure formerly used to alleviate severe intractable pain in cancer patients is called an anterolateral cordotomy. When performed properly, this procedure cuts the axons contained in the lateral spinothalamic tract.
Why are Horse radish peroxidase, alkaline phaosphatase and beta-D-galactosidase enzymes commonly used in ELISA?
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