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You subject a single dsDNA strand to PCR using the PCR protocol discussed during our lab. How many amplified strands of our DNA target would be present after: a. 10 min? b. 30 min? c. 1 hour?
In a generalized transduction experiment, phages are collected from an E. coli donor strain of genotype cys+ leu+ thr+ and used to transduce a recipient of genotype cys- leu- thr-.
What is the dissimilarity between vag and vrg genes? How are BvgS and BvgA involved in regulation of virulence genes? What is a phase variation in B. pertussis and what causes it.
A reactio mixture containing 20 mM O-acetly serine, fifty mM NaCl, 50 mM HEPES buffer (pH 7.6), and O-acetyl serine is incubated at 37 C until the O-acetyl serine is completely hydrolyzed.
Explain the mechanism of horizontal gene transfer during transduction. Describe a disease in which transduction plays a role and explain what it does
Unique Catering wants to issue some 6% preferred stock that has a stated liquidating value of $100 a share. The company has determined that stocks with similar characteristics provide a 12.5% rate of return. What should the offer price be?
What consequences would result from a mutation in the alpha subunit of the G protein that caused its affinity for GDP to be reduced without significantly changing its affinity for GTP?
You are designing a new single-celled organism. Discuss the problems of size, getting molecules like nutrients and wastes in and out, temperature, energy and replication of genetic materials.
Describe the cell-mediated response to the intracellular viruses by starting with an infected epithelial cell. Be sure to include the name of the MHC presenting protein.
Calculate the total number of ATP molecules that will be produced upon complete breakdown of one C18 fatty acid molecule. Which would produce more ATP, this process, or the breakdown of 1 glucose molecule.
Which coenzyme is required for enzymatic transfer of hydride anion in dehydrogenation reaction. How are the fluxes in metabolic pathways established and/or maintained.
In Drosophila, a cross was made between 2-mutant flies, one homozygous for the recessive mutation bent wing and the other homozygous for the recessive mutation eyeless.
What were the contributions of Nestorian Christians, Muslims, and Jews to Medieval Science?
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