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Consider a culture into which two E. coli strains are inoculated. Strain A is normal with g=21 min at 37 degrees C, and 42 mins at 27 degrees C. Strain B has a temperature-sensitive mutation, such that g=55 mins at 37 degrees C, and 39 mins at 27 degrees C.
We inoculate equal amounts of cells (1 ml of each starter culture whose population is 1 x 10^6 cfu/ml) into each of two separate 1 liter volumes of LB broth at time=0. One of the flasks is incubated at 37 degrees C, one at 27 degrees C.Assuming constant exponential growth (i.e. no lag phase, and no exhaustion of nutrients), answer each of these questions:
1) After 6 hours of growth, what is the final concentration of each strain in each flask?
2) What fraction of the total population is Strain A and Strain B in each flask?
3) How long would each flask need to go for one strain to become 90% of the population?
Some proponents argue that the "damage" hypothesis makes more sense from an evolutionary perspective, claiming that it is more advantageous for an organism's defence system to respond to tissue damage than to the mere presence of a foreign microbe. D..
Discuss how does the method used today for exciting action potentials differ from the physiological methods? Does electrical method effect how the action potential is propagated to the next nerve?
You've been experiencing acid indigestion lately, and you'd like a quick fix for the problem. You do a little research on the Internet and discover that your problem is caused by excess stomach acid. In the pharmacy aisles, you investigate various..
In the lab, you will add 4uL of 1M IPTG to 4mL of bacterial culture. What is the final concentration of IPTG in the bacterial culture?
Describe and explain the relationship among the processes of filtration, reabsorption, and secretion in the mammalian kidney.
Write half reactions for the oxidation of 1 molecule glucose and the reduction of oxygen during the aerobic respiration in mammalian cells.
In each of the trials recorded in Table 20-2, contrast the amount of phosphate that moved into an onion plant that is mycorrhizal with the phosphate in an onion plant that is non-mycorrhizal. What conclusion do you reach?
In long-term starvation oxaloacetate is directed away from the CAc and into the gluconeogenesis pathway. Pyruvate starts to be converted to support glucose synthesis while fatty acids are oxidized into acetyl-coA.
find the titer of B-D-galactosidase using the enzyme-linked immunosorbent assay.
Multicellular animals have some mechanism to control cell mass that we do not understand; for in case, salamanders of different ploidies are the same size such that a haploid, a diploid, and a pentaploid organism are about the same size.
What substance is known as the "spreading factor?" How does the "spreading factor" aid an organism in its quest to penetrate the host cell? What is the dissimilarity between net primary production and net primary productivity.
A very important part of the paper is your own opinions and ideas, based on the research you have read. Indicate which sources you agree with and which you do not, and explain your answer.
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