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Consider a culture into which two E. coli strains are inoculated. Strain A is normal with g=21 min at 37 degrees C, and 42 mins at 27 degrees C. Strain B has a temperature-sensitive mutation, such that g=55 mins at 37 degrees C, and 39 mins at 27 degrees C. We inoculate equal amounts of cells (1 ml of each starter culture whose population is 1 x 10^6 cfu/ml) into each of two separate 1 liter volumes of LB broth at time=0. One of the flasks is incubated at 37 degrees C, one at 27 degrees C.
Assuming constant exponential growth (i.e. no lag phase, and no exhaustion of nutrients), answer each of these questions:
1) After 6 hours of growth, what is the final concentration of each strain in each flask?
2) What fraction of the total population is Strain A and Strain B in each flask?
3) How long would each flask need to go for one strain to become 90% of the population?
Snouters are small critters that live on both sides of Ohio River. A population on the north side of the Ohio River is fixed for an allele for blue feet.
A microbiologist found a new restriction endonuclease. When DNA is digested by this enzyme, fragments that average 1,048,500 bp in length are produced.
Compile a list of at least five possible sources of biodiesel (for example, soybeans). You may use on/off-line resources. Please cite where you found the information.
Terry catches a ray-finned fish from the ocean and notices that, attached to its flank, there is an equally long, snakelike organism. The attached organism has no external segmentation, no scales, a round mouth surrounded by a sucker and two small ey..
The malfunction in meiosis where one gamete received two copies of a particular chromosome and the other gamete receives no copies of that chromosome is called?
Complete a chart by identifying six actions that reduce the risk of infection and strengthen the body's immune system. In the second column, explain how the action reduces risk of infection and strengthens the body's immune system.
A man with group A blood marries a woman with group B blood. Their child has group O blood. What are the genotypes? What other genotypes and in what frequencies would you expect offspring from this marriage?
Suggest two different strategies for synthesizing a 0.5-kb gene. Discuss the advantages and disadvantages of both methods.
1. (a)Suppose we wish to determine the structure of a 56-residue protein. Would NMR or X-ray crystallography be likely to be more successful and informative? Why??(b) Same as above, but this time it's a 75 kDa protein.
Describe how protein hormones versus steroid hormones play a role in transducting signals that can affect gene transcription?
In England in the 1700s, Edward Jenner injected a child with a smallpox vaccine that had not yet been tested. After some time had passed, he injected the same child with a living smallpox virus. How do you think Jenner's experimental method differ..
Question: Assume you are working at a corporation that makes poly(ethylene terepthalate) in a batch-type processing. This process requires 2.7kg of ethylene glycol reacted with 9.6kg of terepthalic acid to make the polymer.
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