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Consider a culture into which two E. coli strains are inoculated. Strain A is normal with g=21 min at 37 degrees C, and 42 mins at 27 degrees C. Strain B has a temperature-sensitive mutation, such that g=55 mins at 37 degrees C, and 39 mins at 27 degrees C.
We inoculate equal amounts of cells (1 ml of each starter culture whose population is 1 x 10^6 cfu/ml) into each of two separate 1 liter volumes of LB broth at time=0. One of the flasks is incubated at 37 degrees C, one at 27 degrees C.
Assuming constant exponential growth (i.e. no lag phase, and no exhaustion of nutrients), answer each of these questions:
1) After 6 hours of growth, what is the final concentraion of each strain in each flask?
2) What fraction of the total population is Strain A and Strain B in each flask?
3) How long would each flask need to go for one strain to become 90% of the population?
At equilibrium, where DG=0, the equations can be rearranged to the more familiar form known as the Nernst equation, What is the maximum concentration gradient that can be achieved by the ATP-driven active transport into the cell of an uncharged molec..
In an experiment, you have treated cells with a drug that inhibits the ezymes that place glycosylation trees onto proteins. When you look for you protein, you find it in the rough endoplasmic reticulum but nowhere else. Explain this result.
Determine which of the following could cause a reaction with a positive to become more favorable with respect to making product.
You mistakenly treated each row of data in a dataset as an independent observation. In this case, you would have N = 12 for each of control and treated plants
The R and S loci are 35 m.u. apart. If a plant of genotype RS/rs is selfed, what progeny in what proportions will be seen?
What proportion of progeny would PHENOTYPICALLY resemble.
Recognize precisely which areas of the living human brain are most active in participants who are trying to solve a mathematical problem.
Suppose that you discover a new life form on Mars. These organisms have DNA containing the same four nucleotides as Earth DNA. They also make proteins using a triplet code, so they use the same 64 codons that Earth organisms do.
If humans and shrews shared the common ancestor roughly 80 million years ago, ignoring multiple substitutions at the same site, how several amino acid differences have to be expecting to see between a human and shrew Fibrinogen B sequence.
What new information has the Genome project provided physiologists who study the discipline.
Describe the pathway the restores the supply of NAD+ in muscle tissue when the oxygen supply isn't adequate to meet demands. Write an equation for the process.
Explain why proteins are superior to both RNA and DNA for structural and enzymatic function?
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