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1.) How is PCR superior to colony hybridization for identifying DNA sequences of interest?
2.) What is the natural function of bacterial restriction endonucleases? How might the presence of restriction enzymes affect the use of bacteria as hosts for carrying cloned genes?
3.) Gel electrophoresis analysis of a PCR reaction generated with a pair of oligos showed three fragments instead of the expected single fragment. Give possible explanations for the result.
4.) How would you clone the metabolic genes involved in trp biosynthesis from ecoli?
What are some processes that occur in the nervous system during childhood?
Genes a, b, and c are known to be in that linear order in an organism. A testcross is done that yields 20 double crossover progeny out of a total of 1000 offspring. Assume that there is no interference.
What is the “committed” step of glycolysis? Why is itcalled this? Is this a regulated step?
A 20 year old man injures his right leg in a skiing accident. A Radiograph of leg revels a compund fracture of the right fibula. The leg is immoblized in a cast. Wehn the cast is removed, the pateint notices that acallus has appeared where the cas..
When a snake eats a mouse, what percentage of the energy from the mouse can be used by the snake? Where does most of the rest go?
The normal functioning of any one of these genes is sufficient to convert the colorless compound to the red compound. The abnormal functioning of these genes is designated by a, b, c, respectively.
A man and a woman are both carriers for 2 autosomal recessive disorders, PKU and cystic fibrosis. If they have a daughter, what is the possibility that she will have PKU but not cystic fibrosis.
Explain if bio-remediation is effective, why does oil still persist in Prince William Sound and how do fertilizer work for bioremediation of contaminants such as oil?
Our heart pumping and our circulatory system keep us alive. Many things like poor diet, lack of exercise, drinking and drugs damage our systems, even to the point of early death.
Consider the following two genotypes: AABB, aabb where A and B are dominant and a and b are recessive.
Trace the path of an electron from its origins at the reactioncenter of photosystem II to its final electron acceptor at the endof photosystem I. Be able to name the main molecules involved inthe process (this does not include the individual molec..
What are two cancers that occur predominantly in children(less than 13 years of age), and why is it thought that these cancers occur early in life?
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