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How do you determine the dna fragment sizes

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Reference no: EM131658142

1. During which step of aerobic cellular respiration are most of the high energy co-enzymes, NADH and FADH2, produced?

conversion of pyruvate to Acetyl-CoA

Kreb's cycle

electron transport chain

glycolysis

2. This biochemical test distinguishes Staphylococcus aureus from other species of Staphylococci. What is the name of this test?

oxidase test

indole test

coagulase test

catalase test

3.  ___________ are stretches of DNA that code for functional molecules that are utilized within that organism.

genes

introns

codons

amino acids

4. Which of the following types of RNA is a component of ribosomes?

rRNA

sRNA

tRNA

mRNA

5. This image pictures the blood parasites Trypanosoma brucei and T. cruzi. Identify the structure that is designated by the black arrows and what disease does T. cruzi cause?

nucleus/Chagas disease

kinetoplast DNA/ Chagas disease

kinetoplast DNA/African Trypanosomiasis

nucleus/ African Trypanosomiasis

6. During which step of photosynthesis does carbon dioxide get fixed into sugar molecules?

Calvin-Benson cycle

light reactions

non-cyclic photophosphorylation

cyclic photophosphorylation

7. Which protozoan in Phylum Diplomonada possess 2 nuclei which look like 2 eyes when the parasites are stained for diagnostic purposes?

Leishmania sp.

Acanthamoeba polyphaga

Giardia intestinalis

Trichomonas vaginalis

8.  During an infection with Plasmodium, the gametophytes are produced in the ________________ and sexual reproduction occurs in the _____________.

mosquito/snail

human/mosquito

mosquito/human

snail/human

9. Which retrovirus enzyme do scientist's utilize to synthesize cDNA?

integrase

viral ligase

viral protease

reverse transcriptase

10. Which of the following would you NOT find in a virion?

nucleic acid

capsomeres

protein coat

ribosomes

11. This image illustrates a Kirby-Bauer antimicrobial sensitivity test. The zone of inhibition for TE is 17mm. Using the Table of Interpretive Standards, determine if Escherichia coli is resistant, intermediate or susceptible to TE.

there is not enough information to determine this

susceptible

intermediate

resistant

12. How do you determine the DNA fragment sizes (lengths) once the DNA is separated by agarose gel electrophoresis?

you can guess the size knowing that the largest DNA fragments will travel through the gel the quickest.

Compare the migration of your DNA fragment to a standard which contains known DNA fragment sizes.

Measure the distance that your DNA traveled in the gel and divide by the length of the gel.

Measure the distance that your DNA traveled through the gel, then multiple by 100bp per cm.

Reference no: EM131658142

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