How do you design experiment to test that adenylyl cyclase

Assignment Help Biology

Reference no: EM133380328

Questions:

1. Understanding Background information: In the canonical pathways (pathways that have been discovered in other organisms-not in spiny lobster), Dopamine (DA) modulates potassium channel (IA) in neurons by through several highly conserved dopamine receptors (DR) that belong to the G-protein-coupled receptor (GPCR) superfamily. Type 1 DA receptors (D1Rs) couple with Gs to increase adenylyl cyclase (AC) activity and type 2 DA receptors (D2Rs) couple with Gi/o to decrease AC activity, thereby increasing and decreasing cAMP levels, respectively.

2. Constructing a hypothesis for your own your research: You are working in a molecular biology laboratory to study DA signal transduction pathway in spiny lobster. You already discovered that DA alters rhythmic behaviors (food digestion) of spiny lobsters by modulating potassium channel (IA). You have also identified the presence of D1Rs in a specific type of neuron. What is your hypothesis regarding to how DA modulate potassium channel (IA)?

3. Experiment design 1: How do you design experiment to test that adenylyl cyclase (AC) is involved in DA modulation of IA. Forskolin, which is a chemical that can mimic the effect of DA is available in the lab. 4. Experiment design 2: How do you design experiment to test that protein

Reference no: EM133380328

Questions Cloud

Is the risk that the child develops cmd 25 percent : have a child with a carrier of CMD due to a mutation in a collagen gene. Is the risk that the child develops CMD 25 percent? Why or Why not

What is aumanns agreement theorem : What is Aumann's Agreement Theorem? What is the Myerson-Satterthwaite Theorem, and how might it explain the existence of conflict between rational actors?

What are the experimental steps required to take : What are the experimental steps required to take to construct pFXN-AcGFP1-N1 using standard molecular biology techniques and E. coli as the host

What is the primary characteristics of this disorder : Cystic Fibrosis is the result of recessive gene. What is the primary characteristics of this disorder as is relates to the lungs.

How do you design experiment to test that adenylyl cyclase : How do you design experiment to test that adenylyl cyclase (AC) is involved in DA modulation of IA. Forskolin, which is a chemical that can mimic the effect

What is difference between a cosmic war and regular war : How do you galvanize people against the foe/enemy? Describe the role of each: dehumanization, depersonalization, and satanization.

Could a fox breeder establish a true-breeding variety : In foxes, a pair of alleles interact as follows: PP is lethal; Pp produces a platinum-coloured fur, and pp produces silver foxes. Could a fox breeder establish

How many bacterial cells or ml were in the original culture : You then spread 1 ml of this final broth tube onto a plate and count 30 colonies. How many bacterial cells/ml were in the original culture?

What ratio of living progeny (white to colored) is expected : Half of the progeny from this cross are white, half are horses of other colors. If one selects from these progeny two white horses and crosses them, what ratio

User Account

All Pages