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You believe that the cytosolic protein p007 is involved in mediating growth hormone-stimulated proliferation in cells. You wish to establish a cell model using your cultured fibroblasts. Provide clear explanations to the following.
a) Describe how you would transfect the growth hormone receptor DNA into your cells to create stable cell lines, and include a description of the benefits of using stable cell lines versus transiently transfected cell line.
b) Describe how you would measure specific receptor binding of GH to your stable cell line.
c) Describe how you would measure growth hormone-induced proliferation in your stable cell line. Make sure to include controls.
d) Describe how you would use RNAi to demonstrate the requirement for p007 in growth-hormone stimulated proliferation. Make sure to include controls.
You buy a new house and the front yard has some really nice rose bushes, but they are surrounded by lots of weeds! You don't really want to try and dig them out by hand and your nice new neighbor comes over with a bottle of weed killer.
consider a protein that has the following sequence of amino acid. this is the wild type protein sequence. met - thr -
oThe major structural and functional dynamics (processes) of that ecosystem including change over time oHow humans may have affected biogeochemical cycles in that ecosystem, including impacts to the nitrogen, phosphorus, or carbon cycle
What is the “committed” step of glycolysis? Why is itcalled this? Is this a regulated step?
Give the other component that makes up the cell membrane apart from the protein layer?
Discuss why glycolysis is called an anaerobic pathway, i.e., why (and how) glycolysis can continue to run under anaerobic conditions. (Please do not discuss the Cori Cycle in your response).
Write down a paper describing the scientific method and the fundamentals of research. Address each of the following in your paper:
When gold beads were injected into a cell to see whether or not they could get into the nucleus itwas discovered that beads bigger than 9 nm in diameter (orbigger than the size of a proteinwith a molecular weight of ~40,000) could not get intothe ..
1 in 2010 under its watersense program the epa began promoting showerheads that produce still-lower flows of 2 gallons
List the goals of medical nutrition therapy for your patient and suggest an appropriate diet. Does your patient/client require a dietary modification of their regular diet?
Imagine that you are a marine biologist. As part of your exploration you dredge up an unknown animal from the seafloor. Describe some of the characteristics you should look to determine the phylum to which the creature should be assigned.
Why did you have to flip the coin twice to determine hair and eye color? Show the cross of a wavy haired person with a wavy haired person by diagraming a Punnett square.
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