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You believe that the cytosolic protein p007 is involved in mediating growth hormone-stimulated proliferation in cells. You wish to establish a cell model using your cultured fibroblasts. Provide clear explanations to the following.
a) Describe how you would transfect the growth hormone receptor DNA into your cells to create stable cell lines, and include a description of the benefits of using stable cell lines versus transiently transfected cell line.
b) Describe how you would measure specific receptor binding of GH to your stable cell line.
c) Describe how you would measure growth hormone-induced proliferation in your stable cell line. Make sure to include controls.
d) Describe how you would use RNAi to demonstrate the requirement for p007 in growth-hormone stimulated proliferation. Make sure to include controls.
Colchicine inhibits the formation of the spindle fibers. Your instructor is interested in creating a polypoid hybrid between cucumbers and cantaloupe. Describe the steps he would have to go through to get this polyploidy. Include in your descripti..
question 1 hospital ownership can be not-for-profit for-profit or governmental. based upon your own experiences and
Based on the absorption spectrum of bradford dye why is 595nm the best wavelength to measure protein concentration with bradford dye? I know one of the reasons is simply because the difference between the two forms of the dye is the greatest at..
how is the stem of a water lily different from the stem of a dandelion and how does te stem of a water lily help it live in a pond. ?
Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity. (Recall: enzymes are proteins.) To complete this project, it may be useful for you to review the Scientific Method Tutorial (found the Course Content ..
find which of the following describes the type of the reaction(s) catalyzed: condensation (carbon-carbon bond formation); dehydration (loss of water); hydration (addition of water); decarboxylation (loss of CO2); oxidation-reduction; sutrate-level..
What types of molecules would I find in functional goup of thecarboxyl? i have looked thru the book and cant see what theymight be other then fatty acids
How we can use nutrigenomics to overcome free radical damage in DNA in order to prevent a biological system against to cancer?
Is water a polar or a non-polar molecule? What is the consequence of that characteristic for the function of water as solvent?
Name the five theoretical approaches to child development. give a brief description of each approach - define DNA and briefly address where it is found, how it works, and its construction.
When Rh-negative women is ABO compatible w/ her husband, the risk of RH alloimmunization is GREATER than when they are ABP incompatible; why the difference?
A double-stranded fragment of viral DNA, one of whose strands is shown below, encodes two polypeptides called vir-1 and vir-2. Adding this double stranded DNA fragment to an in vitro transcription and translation system yields peptides of 10 residues
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