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Question: Scientists can use mutants to study metabolic pathways. These organisms have a mutation in a gene encoding a metabolic pathway enzyme that results in the inactivation of that enzyme. Saccharomyces cerevisiae (a yeast species) mutants are available that grow on fructose, but not glucose. Which of the following glycolytic enzymes is mostly likely affected?
1. phosphoglycerate kinase2. phosphoglycerate mutase3. pyruvate kinase4. phophoglucose isomerase (phosphohexose isomerase or glucose-6-phosphate isomerase)5. enolase
The interaction between bases on different strands of DNA molecules depends somewhat on the identities of neighboring bases. For example, the melting temperature of an oligonucleotide
GTP is again added to the redissolved pellet proteins, and the solution is again warmed for a few minutes. Then centrifugation at similar speed described above is again performed. This process is repeated several times.
Discuss the difference between the Law of Segragation and the Law of Independent Assortment? During which phases of meiosis do these occur?
however normal E.coli cells can synthesize all the amino acids, some mutants called amino acid auxotrophs, are unable to synthesize specific amino acids and require the addition of that amino acid to the culture medium for optimal growth.
In a herd of Shorthorn cattle the frequency of the r allele is .4. Because of prevalence of pinkeye, white cows produce and average of 0.60 calves that reach puberty.
select a chromosomal disorder from the March of Dimes Foundation Website.
Studying and predicting results of experiment conducted with mutant mouse. A researcher recently found out a mutant mouse with an interesting T cell phenotype.
A mixture of very hot and very cold water. Organize them, so your right hand is in the front of cold water, your left hand is in front of hot water, and lukewarm water is in the middle.
Many infected animals are induced by the parasitic microsporidians to develop huge cells, known as xenomas, which are full of spores. Given their large size, what must be true of the xenomas.
compute how many molecules X and Y the cell contains at any time. Which molecule would be preferred for rapid signaling? Why?
Assume you are analyzing the function of an unknown drug that you suspect alters metabolism in human cells. You add the drug in different amounts in a colony of cells and find that above a certain concentration,
What is most likely genotype of the father? Mother? If the seventh child had blue eyes what could you conclude about parents genotyoes. What possible dissimilarity between these forms would give chemists a clue that open-chain form was not present.
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