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1. Assume you are looking for a particular pathogen in a sample and you are using a highly selective medium, and so you make five 1:10 dilutions of the sample and you plate 0.2 mLs of each dilution. The following day you find that you have 80, 50, 25, and 1 colonies on plates 2, 3, 4, and 5. How many pathogens per mL do you have?
2. You have 1mL containing 10^8 cells and you need 2.5x10^7 cells. What do you do? (how many cells in each 0.1mL)
3. You have 10 mL of 10^6 cells/mL prep and you need 1mL of 10^7 cells. What do you do? Hint: you can se cetrifugation, but then you have to perform another step)
4. You are generating a vaccine prep that contains 10^8 microbes per mL and you need to know the ID50 of your prep. So you run a challenge study with 50 mice where you inoculate each mouse with 50 uL of the prep. Within 3 days you record the number of sick mice and you find that 30 of the mice are sick. How can you estimate the ID50, and what is that estimate? (use a ration)
Using Rasmol produce an image of oxidoreductase structure code 2XXE that, clearly illustrates the location of the catalytic site of the enzyme. State and show in an image the active site residues.
Propose two ways the photosynthesis process be improved. If the sequence TCGTA was used as a template in DNA replication, what will the sequence of bases be on the newly synthesized strand.
The main ecological imbalance on Earth is between the human species and the rest of Earth's biota. Determine two specific actions we could do to achieve a balance
Explain two ways that mutations can affect the phenotypes that does not include alteration of the protein coding sequence.
You start to have a scratchy feeling in the back of your throat. After a few days it hurts to swallow, so you go to doctor. The doctor tells you that you have strep throat.
A patient lost a lot of blood during surgery and his blood pressure dropped from 120/80 to 90/50. Describe how the kidneys respond to this change in blood pressure.
DNAase is an enzyme that catalyzes the hydrolysis of covalent bonds that join nucleotides together. What will first happen to DNA molecules treated with DNAase.
The pH of the inner thylakoid space has been measured, as have the pH of the stroma and of the cytosol of a particular plant cell. Which, if any, relationship would you expect to find.
Estimate joints are moving in a forward lunge and what muscles are involved? What type of movement is occuring.
Describe why you can predict that meiosis gives rise to genetic differences between parent's cells and daughter cells in fewer cell divisions than mitosis does.
As Jung-Min Kee and Tom W. Muir noted in their recent review article, 2012 marked the fifty anniversary of discovery of histidine phosphorylation in proteins.
List the different hypothalamic regions and peripheral signals involved in the control of feeding and satiety.
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