Generate the transducing phage

Reference no: EM13122142

Time-of-entry experiments indicate that a gene for resistance to kanamycin, kan-r, is closely linked to a gene for resistance to bacteriophage T5, T5-r. A geneticist has strains of two genotypes available, kan-r T5-s (resistant to kanamycin, sensitive to phage T5) and kan-s T5-r (sensitive to kanamycin, resistant to phage T5). In an experiment using phage P1 to learn whether the genes are close enough for cotransduction, how would the experiment be done?

Part A: Specifically, which strain would you use to generate the transducing phage and which stain would you infect with this phage?

Part B: What methods would you use to determine if the genes had been co-transduced in the recipient cell?

Reference no: EM13122142

Questions Cloud

How long it take for partial pressure drop of cylclopropane : If a 2.1 L reaction vessel initially contains 723 torr of cyclopropane at 485 degrees C , how long will it take for the partial pressure of cylclopropane to drop to below 105 torr ?

Loss contingency accrued in a company financial statements : A loss contingency shoulf be accrued in a company's financial statements only if the likelihood that a liability has been incurred is:

Primes and divisibility word problem : When the accountants for lose-a-digit Computer, Inc. had finished preparing their annual budget, they presented the final figures to the president, I.M. Smart.

Conduct a hypothesis test using the p-value method : Reccomend asprin for their patients with children Conduct a hypothesis test using the P-Value method. What conclusions would you make when a=o.2 and a=0.02.

Generate the transducing phage : What methods would you use to determine if the genes had been co-transduced in the recipient cell?

I''m not a good writer : A fellow student says, "So, I'm not a good writer. But I have other places to put my study time. I'm a management major. I'll have secretaries to handle my writing for me." Give this student your best advice, including the reasoning behind it.

Suppose a machine has three independent components : Suppose a machine has three independent components with Exp(.1) lifetimes. Compute the expected lifetime of the machine if it needs all three components to function properly

Describing how you would market the product : Your Industry has just developed a new sports drink that is in a container which will keep it cool for up to 6 hours. Develop a marketing plan for this product and then write a 3 page paper describing how you would market the product.

Explain how governments use monetary and fiscal policy : Explain how governments use monetary and fiscal policy to manage the economy and use technology and information resources to research issues in principles of economics.

User Account

All Pages