Generate a genetic system for gram-positive

Assignment Help Biology
Reference no: EM13748014

Test Problem Set:

1. Generate a genetic system of:

a) inactivation
b) transfer 

of any E. coli. gene selected by Dr. Roberts, and using transduction

Imagine Dr. Roberts telling you: "Student, I need to inactive gene X of E. coli using transduction" or "I need to transfer the gene X from this E. coli to another, by transduction"

2. Generate a genetic system for gram-positive that (based on the genetic material transference mechanisms):

a. That allow us to know that the bacterial community is conducting quorum sensing.

b. That allows that during the logarithmic phase, the bacteria eliminates the bacteria A (who is sensitive to Chloramphenicol); and during the stationary phase, the bacteria eliminates the bacteria B (who is sensitive to Tetracycline).

3. A bacteria posses a plasmid with: resistance gene to chloramphenicol (cat), an indispensable gene for starch degradation (amiA), and another gene for lactose degradation (lac Z). For this bacteria, the genetic transference systems and IS (insertion sequences) are unknown. Design and demonstrate, making sure to include specific verification systems:

a. A bacteria able to transfer lacZ gene via conjugation

b. Development of bacteriophages capable of transferring amiA gene or a portion of amiA gene via transduction

4. Design an experiment that allows me to determine if a pair of genes or isolated DNA fragments, encodes for the following (bioinformatics, Southern, antibodies or probes can't be used):

a. ClpC vs λdgal

b. degS vs TraI

5. Develop and present an ideal plasmid that could be used for Dr. Smith, Dr. Rose and Dr. Spencer investigations. Remember to use plassmapper (free program to create the image/figure of the plasmid).

Reference no: EM13748014

Previous Q& A

  Equal amounts over the next

Can you please show me how to do this in ms Excel. Find the payment you would need to make per year at a discount rate (interest rate that the bank charges you) from 0% to 50% (51 answers), if you borrow $5,000,000 ($5 million) today (PV) and pay ..

  Significant economic crisis in the recent history

Risk management negligence within the financial services industry contributed to one of the most significant economic crisis in the recent history of the U.S. During this time, Lehman Brothers, a global financial services company, filed for bankru..

  What was the main idea behind the monroe doctrine

What was the main idea behind the monroe doctrine? the british must stop the main increasing sailors. spain must surrender florida to the united states.

  Describe at least three factors that affected the resolution

Describe at least three factors that affected the resolution of the problem. What is the main goal of the character you are analyzing? Support your response.

  What tool would archaeologist use to investigate citys ruins

What tools would archaeologist and historians use to investigate this city's ruins? What types of artifacts or evidence would historians and archaeologist look for to determine what life was like for the people of the time?

  What is the present cost of the machine

What is the present value of the return at 3% and what is the present cost of the machine if the interest rate is 3%?

  How many different types of gametes can be generated

How many different types of gametes can be generated by an individual with genotype AaBB

  Symbols of culture are called artifacts

Symbols of culture are called artifacts. Artifacts are the most visible and accessible level of culture. These include behaviors, stories, rituals (everyday practices that are repeated frequently), and symbols

  Foreign direct investment opportunities

Demonstrate your ability to provide reasoning for your response to discussion questions - demonstrate your ability to provide reasoning for your response to discussion questions.

  Find five examples of the renaissance legacy around you

Find five examples of the Renaissance legacy around you and explain in two to three sentences why you think each one is a good example. Make sure to include items from each category: art, architecture, literature, and inventions.

Reviews

Write a Review

 

Similar Q& A

  Assume one beta-globin gene per haploid genome

The PCR recommends that you start with 25 ng of human DNA. You have purified 25ng of genomic DNA from human white blood cells. If the human haploid genome contains 3 x 10^9 bp of the beta-globin gene are present in 25ng of DNA? assume 1 beta-globin g..

  Describe the animal and plant evolution

The following type of mechanism of evolution is given: This mechanism of evolution is ANIMAL AND PLANT EVOLUTION (such as hox genes, gene duplication

  Create a concept map using the following terms

How do you create a concept map using the following terms to show the process of digestion with these terms bile, chemical digestion, chyme, digestion, molar, pancreas, saliva, small intestine, and stomach?

  The shape of a protein is frequently very important

What do you think would happen if a person had a mutation that prevented the jaw part of their HLA molecules from becoming the correct shape.

  Hypertonic or hypotoni

Hypertonic, or hypotonic? Would water move into or out of the red blood cells (RBC) to maintain equilibrium?

  What is a potential cascading effect to everglades flora

What would be a potential cascading effect to the Everglades flora and fauna as a result of interspecific competition.

  Is wind power green

You are required to critically analyze one of the following five (5) environmental issues that have been addressed in Contemporary Environmental Issues, Is wind power green

  What happened to the carbon dioxide the animals produced

Using the respirometer-manometer, you observed the amount of oxygern being used by animals in a closed chamber. What happened to the carbon dioxide the animals produced while in the chamber?

  Which commonly applies to hospitality industry

What are the disadvantage and advantage of each and which commonly applies to hospitality industry. How is this caused by the sympathetic nervous division.

  Question about bacterial mutation

Assume you leave a culture inthe 37 C incubator with out tryptophan for week instead of overnight like you planed. to your surprise culture has shown some visible growth so you reinoculate from that flask into a new flask

  Which component of the cytoskeleton is most important

Which component of the cytoskeleton is most important in.

  Population at hardy weinberg equilibrium

Determine what genotype frequencies would you expect to see in the next generation if there is no evolution.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd