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Assignment -
In this assignment need help with crucial forensic analysis of archaeological human remains found in a new site in North Queensland. We have sampled these remains and sent them for a full mitochondrial DNA sequencing and we would like to understand their origins. We have also generated in the same way a reference panel of 100s of reference datasets from various existing human volunteers. Our team sent them to a "sequencing centre" and seems we got more than we bargained for.
They tell us that they did Next Generation Sequencing using Paired End libraries with a fragment size of 400 base pairs and gave us the raw data.
It seems the data has been provided to us in a peculiar format. It does not really work with Excel! That is when we realised we should write to a bio-informatician such as yourself. Unfortunately, we are not experts in your bioinformatic science and do not know what to do. Could you please help us by downloading these data and comparing the archaeological sample labelled X with the reference samples numerically numbered sequentially 1, 2, 3 etc? Then, please tell us which subgroup does X belong?
We used the mtDNA because it is so conserved and likely to be amenable to laboratory methods. Because this forensic sample X is so old, we feel it would be prudent if you undertook a quality control of the data (e.g. the assembled data) and tell us if it has all the 'genes' that we expect. If it does not, then we need to know (e.g. using a diagram or other graphic) so we can re-assess our laboratory methods or at least put the results within a context.
Note - Need to use python jyputer.
Attachment:- Genome Assignment File.rar
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