Five step protocol

Assignment Help Biology
Reference no: EM1389694

Using a five step protocol, you have purified an enzyme to the point where the specific activity remains constant when you perform any additional purification steps. When you run the purified enzyme on a gel filtration chromatography column, you find it has a molecular mass of 50 kD. However, when you run the purified enzyme on a SDS-PAGE gel, you obtain two different sized peptides one 30 kD and the other 20 kD. What could be the possible explanations for this result?

Reference no: EM1389694

Questions Cloud

Components of the reticuloendothelial system : Define the complement cascade and there are two infection-fighting results of this cascade. What are they and discuss the main components of the reticuloendothelial system
Determine the critical value for the test : the null hypothesis is to be tested at 95% confidence. Determine the critical value(s) for this test.
What is the additional holding cost on account : what would be the ROP and the associated safety stock. What is the additional holding cost on account of the safety stock.
Determine length of the newly transposed copia element : A Drosophila P element 2.2 kb in length is modified by adding a 0.8-kb intron sequence to one of its exons. A copia element of 5.2 kb is modified through adding the same 0.8-kb intron to its central region.
Five step protocol : Using a five step protocol, you have purified an enzyme to the point where the specific activity remains constant when you perform any additional purification steps.
Specific mwr program : As a result of these actions, explain the "who, what, when, where and why" of how you would add new life into a specific MWR program to increase and continue its success?
City enjoy shopping online : A survey found that 85% of women in a given city enjoy shopping online. Suppose you randomly selected 6 women from the city and ask them whether or not they enjoy shopping online. Find the probability that exactly 4 say they enjoy shopping online.
The number of no-shows is normally distributed : The number of no-shows is normally distributed with a mean of 50 and standard deviation of 15. If a customer with booking cannot be given a room, NLI reimburses them with cash equal
Dna mutation in a gene : Determine what kind of DNA mutation in a gene would result in the production of a shorter version of a protein missing amino acids in the middle of the protein

Reviews

Write a Review

Biology Questions & Answers

  Question about animal behavior

Satellite and attached male horseshoe crabs do not have the same reproductive success. why hasnt sexual selection eliminated the low-payoff.

  Which would show that the two populations are different

A storm brings two formerly separated populations of beetles together. They look very similar. Under the biological species concept, which would show that the two populations are different species.

  Discuss the major types of histone proteins

Discuss the five main types of histone proteins, and describe what role each of them plays in the nucleosome. Based on what you know about them,

  Medications administration routes

Why is oral administration of a medication the most desirable route? Why can some medications not be given orally? What medications can be divided for doses? What medications should not be crushed for administration?

  The differences between the two forms of phylum cnidaria

Utilize the words mesoglea, epidermis, gastrodermis, polyp, medusa, and gastrovascular cavity to describe the differences between the two forms of phylum cnidaria.

  Rostral caudal axis

If you generated a mutation that sped up rate at which the node moves toward the posterior, what effect would it have on somite formation?

  Major evolutionary step in the history of life

Life arose in water.  Explain w hy do you think colonization of land represented a major evolutionary step in the history of life?

  Enzyme aldehyde dehydrogenase

It is the accumulation of acetyaldehyde that results in flushed face, dizziness, and light headedness responses that are associated with ethanol intoxication.

  The composition of diets and living conditions were alike

Blood glucose was monitored weekly for all mice. After five weeks, there was no change in average body weight between groups.

  Find the cause for the symptoms

Assume your friend Charles D., is a forty-five year old male who is newly divorced and is living by himself in a new home. He has told you about some health issues including weight loss.

  Explanation of thigmotaxy in mice

Explain why is it important to use animals that are the same sex and approximately the same age in experiements?

  Identify the solution inside bag washypertonic

Complete each of statements given below to identify whether the solution inside each bag washypertonic, hypotonic or isotonic.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd