Find cell density of cell suspension

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Reference no: EM1385935

Question: In order to perform a fluorescence experiment, you need to seed a 4-well chamber slide with 60,000 cells in each well. To prepare for the cell count, you transferred 20 uL of cell suspension into 30 uL of trypan blue. You calculated 19, 12, 25, 29 and 11 cells in 5 large boxes using the Neubauer hemacytometer. Based on this information, what is the cell density (cells per mL) of your cell suspension? How much cell suspension will you need to transfer roughly 60,000 cells into to each well?

Reference no: EM1385935


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